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. 2022 Aug 7;11(15):2063.
doi: 10.3390/plants11152063.

Analysis of Chlorogenic Acid in Sweet Potato Leaf Extracts

Chun-Hui Chiu  1   2 , Kuan-Hung Lin  3 , Hsin-Hung Lin  4 , Wen-Xin Chu  5 , Yung-Chang Lai  6 , Pi-Yu Chao  7
Affiliations

Analysis of Chlorogenic Acid in Sweet Potato Leaf Extracts

Chun-Hui Chiu et al. Plants (Basel). .

Abstract

Sweet potato (Ipomoea batatas L.) is one of the most important food crops worldwide, with leaves of different varieties showing purple, green and yellow, and these leaves provide a dietary source of nutrients and various bioactive compounds. The objective of this study was to identify the active constituents of chlorogenic acids (CGAs) in different methanolic extract of leaves of three varieties of sweet potato (purple CYY 98-59, green Taoyuan 2, and yellow CN 1927-16) using liquid chromatography-tandem mass spectrometry. Genotype-specific metabolite variations were observed; CGAs and three isomeric peaks were detected in sweet potato leaf extracts (SPLEs). Among them, the yellow SPLE contained the highest contents of 3,5-dicaffeoylquinic acid (3,5-di-CQA) and 3,4-dicaffeoylquinic acid (3,4-di-CQA), followed by the green SPLE, whereas the purple SPLE retained lower 3,5-di-CQA content compared to yellow and green SPLEs. All three SPLEs contained lower 4,5-dicaffeoylquinic acid (4,5-di-CQA) and CGA contents compared to 3,5-di-CQA and 3,4-di-CQA, although CGA constituents were not significantly different in genotypes, whereas purple SPLE contained higher 4,5-di-CQA content compared to yellow and green SPLEs. This study indicates that SPLs marketed in Taiwan vary widely in their biological potentials and may impart different health benefits to consumers.

Keywords: chlorogenic acids; liquid chromatography-tandem mass spectrometry; sweet potato leaf.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structure of chlorogenic acid (CGA) and isochlorogenic acids, 3,5-dicaffeoylquinic acid (3,5-di-CQA), 3,4-dicaffeoylquinic acid (3,4-di-CQA), and 4,5-dicaffeoylquinic acid (4,5-di-CQA).
Figure 2
Figure 2
LC-MS/MS analysis of chlorogenic acid (CGA, peak 1), 3,4-dicaffeoylquinic acid (3,4-di-CQA, peak 2), 3,5-dicaffeoylquinic acid (3,5-di-CQA, peak 3), and 4,5-dicaffeoylquinic acid (4,5-di-CQA, peak 4).
Figure 3
Figure 3
Content (μg/g DW) of chlorogenic acid (CGA), 3,5-di-CQA (3,5-dicaffeoylquinic acid), 3,4-di-CQA (3,4-dicaffeoylquinic acid), and 4,5-di-CQA (4,5-dicaffeoylquinic acid) of purple (P, CYY 98-59), green (G, Taoyuan 2), and yellow (Y, CN 1927-16) leaf extracts. All MS/MS data for analytes were collected in the multiple reaction monitoring (MRM) mode, using MassLynx v4.1 software. The concentrations of CGAs were expressed as means ± standard deviations (n = 3). Means with different letters among the three genotypes of sweet potato leaves indicate statistical significance at p < 0.05.
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