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Review
. 2022 Jul 26:13:941532.
doi: 10.3389/fmicb.2022.941532. eCollection 2022.

Coliphages as viral indicators of sanitary significance for drinking water

Suniti Singh  1 , Robert Pitchers  2 , Francis Hassard  1   3
Affiliations
Review

Coliphages as viral indicators of sanitary significance for drinking water

Suniti Singh et al. Front Microbiol. .

Abstract

Coliphages are virus that infect coliform bacteria and are used in aquatic systems for risk assessment for human enteric viruses. This mini-review appraises the types and sources of coliphage and their fate and behavior in source waters and engineered drinking water treatment systems. Somatic (cell wall infection) and F+ (male specific) coliphages are abundant in drinking water sources and are used as indicators of fecal contamination. Coliphage abundances do not consistently correlate to human enteric virus abundance, but they suitably reflect the risks of exposure to human enteric viruses. Coliphages have highly variable surface characteristics with respect to morphology, size, charge, isoelectric point, and hydrophobicity which together interact to govern partitioning and removal characteristics during water treatment. The groups somatic and F+ coliphages are valuable for investigating the virus elimination during water treatment steps and as indicators for viral water quality assessment. Strain level analyses (e.g., Qβ or GA-like) provide more information about specific sources of viral pollution but are impractical for routine monitoring. Consistent links between rapid online monitoring tools (e.g., turbidity, particle counters, and flow cytometry) and phages in drinking water have yet to be established but are recommended as a future area of research activity. This could enable the real-time monitoring of virus and improve the process understanding during transient operational events. Exciting future prospects for the use of coliphages in aquatic microbiology are also discussed based on current scientific evidence and practical needs.

Keywords: F+ coliphage; coliphage; drinking water quality; drinking water treatment; online monitoring; somatic coliphage.

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Conflict of interest statement

The Water Research Centre received funding from the United Kingdom Water Industry Research. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, and the decision to submit it for publication. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Schematic representing coliphages as viral indicators of sanitary significance for drinking water. Each panel represents a different example of this field. (A) Phage multiplication in environmental water matrices, (B) fate and transport of phages in drinking water treatment plant, and (C) inline sensors for phage detection and monitoring for disinfection optimization and risk assessment.
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