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. 2022 Aug 1:2022:3678240.
doi: 10.1155/2022/3678240. eCollection 2022.

Study of the Antimicrobial Activity of the Chinese Dong Ethnic Minority Medicine, Madeng'ai

Zhenrong Tang  1   2 , Yannan Zhao  1 , Zaiqi Zhang  3 , Huan Yue  1 , Dan Wang  1 , Shengchun Liu  2 , Hua Tang  1
Affiliations

Study of the Antimicrobial Activity of the Chinese Dong Ethnic Minority Medicine, Madeng'ai

Zhenrong Tang et al. Evid Based Complement Alternat Med. .

Abstract

The overuse of antibiotics has contributed to the emergence of multidrug-resistant bacteria, which poses a challenging task for clinical therapy. Thus, new agents with antibiotic efficacy against multidrug-resistant infections are needed. The traditional Dong ethnic minority medicines have emerged as a new source for prodrug selection. Among them, Madeng'ai (PotentillafreynianaBornm) is widely used by the folk for anti-infection and wound healing, although the mechanisms remain unclear. In this study, the antimicrobial activities of Dong medicine Madeng'ai were evaluated both in vitro and in vivo. S. aureus, E. coli, E. faecalis, P. aeruginosa, K. pneumoniae, and A. baumannii were cultured in LB media, different concentrations of Madeng'ai powder solution were added to the LB agar plates to evaluate minimal inhibitory concentration. An animal study was performed on a mouse excisional wound model combined with bacterial solution injection in the wound area. After Madeng'ai or PBS treatment, hematoxylin and eosin analysis were used for pathological analysis of skin tissues from the infected area. Madeng'ai powder solution over 2 mg/mL concentration completely inhibited E. coli growth. At 4.0 mg/mL, Madeng'ai significantly inhibited the growth of E. faecalis, Pseudomonas aeruginosa (PAE), Klebsiella pneumoniae, and Acinetobacter baumannii. The mouse model revealed that Madeng'ai could suppress the growth of MRSA and PAE and accelerate healing of cutaneous wounds. Madeng'ai, a newly discovered Dong ethnic minority medicine possesses considerable antimicrobial activity against both human normal pathogenic bacteria and multiresistance bacteria such as Pseudomonas aeruginosa, S. aureus, and Acinetobacter baumannii. Therefore, Madeng'ai has great potential for further study and clinical application.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Morphology of Madeng'ai, a variant of Potentilla freyniana Bornm (lower panel) and Potentilla freyniana Bornm (upper panel).
Figure 2
Figure 2
(a) MIC results of Madeng'ai against S. aureus and E. coli. (b) The number of colonies on plates of different concentrations of Madeng'ai against S. aureus and E. coli. ∗∗∗p < 0.01 versus blank control group.
Figure 3
Figure 3
(a) The inhibitory effect of Madeng'ai against E. faecalis, P. aeruginosa (PAE), K. pneumoniae, and A. baumannii. (b)The number of colonies on plates of E. faecalis, P. aeruginosa (PAE), K. pneumoniae, and A. baumannii after 4 mg/ml Madeng'ai treatment. ∗∗∗p < 0.01 versus blank control group.
Figure 4
Figure 4
The effects of Madeng'ai on bacterial growth and histological and body change of methicillin-resistant S. aureus-infected mice. (a) Recovery of methicillin-resistant S. aureus-infected wounds on BALB/c mice after Madeng'ai or PBS treatment. (b) LB agar of methicillin-resistant S. aureus growth from infected wound secretions after treatment with Madeng'ai or PBS. (c) H&E analysis of skin tissues from wounds infected with methicillin-resistant S. aureus after treatment with Madeng'ai or PBS. (d) Changes in mouse body weight during treatment.
Figure 5
Figure 5
The effects of Madeng'ai on bacterial growth and histological and body change of PAE-infected mice. (a) Recovery of PAE-infected wounds after treatment with Madeng'ai or PBS (control). (b) The growth of PAE from wound secretions of infected mouse. (c) H&E analysis of skin tissues from wounds infected with PAE after treatment with Madeng'ai or PBS (control). (d) Changes in mouse body weight during treatment.
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References

    1. McEwenand S. A., Collignon P. J. Antimicrobial resistance: a one health perspective. Microbiology Spectrum . 2018;6(2):6–2. - PMC - PubMed
    1. Brinkac L., Voorhies A., Gomez A., Nelson K. E. The threat of antimicrobial resistance on the human microbiome. Microbial Ecology . 2017;74(4):1001–1008. - PMC - PubMed
    1. Ferri M., Ranucci E., Romagnoli P., Giaccone V. Antimicrobial resistance: a global emerging threat to public health systems. Critical Reviews in Food Science and Nutrition . 2017;57(13):2857–2876. doi: 10.1080/10408398.2015.1077192. - DOI - PubMed
    1. Spellberg B., Blaser M., Guidos R. J., Boucher H. W., Gilbert D. N. Policy Paper. Combating antimicrobial resistance: policy recommendations to save lives. Clinical Infectious Diseases An Official Publication of the Infectious Diseases Society of America . 2011;52(Suppl 5):p. S397. - PMC - PubMed
    1. Xiao Y., Li L. A national action plan to contain antimicrobial resistance in China: contents, actions and expectations. AMR Control . 2017;15(3):17–20.

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