Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jul-Aug;26(4):348-352.
doi: 10.4103/jisp.jisp_80_21. Epub 2022 Jul 2.

Matrix metalloproteinase-9-1562 C/T promoter gene polymorphism in chronic periodontitis: A cross-sectional observational study

Puja Sarkar  1 , Thamil Selvan Muthuraj  2 , Prasanta Bandyopadhyay  3 , Papita Ghosh  4
Affiliations

Matrix metalloproteinase-9-1562 C/T promoter gene polymorphism in chronic periodontitis: A cross-sectional observational study

Puja Sarkar et al. J Indian Soc Periodontol. 2022 Jul-Aug.

Abstract

Background: Matrix metalloproteinases (MMPs) are a major group of enzymes, released in inflamed periodontal tissues in large quantities, resulting in connective tissue matrix breakdown. One of the most predominant MMPs is MMP-9. Association between chronic periodontitis (CP) and MMP-9 gene polymorphism (GP) in some ethnic populations has been already established. The aim of the current study was to assess the association of single-nucleotide polymorphism in the promoter region of MMP-9 gene with CP in Kolkata population, if any.

Materials and methods: Forty eligible individuals were recruited for the study and grouped as A and B: Group A (twenty periodontally healthy individuals) and Group B (twenty CP patients). Venous blood samples collected from all the forty individuals were used to isolate the genomic DNA. GP for MMP-9 gene was detected using polymerase chain reaction and sequencing method. The data obtained were then analyzed statistically.

Results: A significantly different genotypic and allelic variation was observed in the control and test groups, with C/T genotype and T allele present in only CP group.

Conclusion: Within the limitations of this study, we can conclude that there was no significant association between patients with periodontitis and MMP-9-1562 C/T promoter GP.

Keywords: 92-kDa Type IV collagenase; 92-kDa gelatinase; chronic periodontitis; gelatinase B; genetic polymorphism; matrix metalloproteinase-9.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Flowchart showing the study population. GA = Group A; GB = Group B; n = number of study participants
Figure 2
Figure 2
(a) Sequencing result with C/C genotype; (b) Sequencing result with C/T genotype. A – Adenine; G – Guanine; C – Cytosine; T – Thymidine
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Glossary of Periodontal Terms. American Academy of Periodontology. [Last accessed on 2020 Apr 18]. Available from: https://members.perio.org/libraries/glossary/entry?GlossaryKey=961be2e7-... .
    1. Nunn ME. Understanding the etiology of periodontitis: An overview of periodontal risk factors. Periodontol 2000. 2003;32:11–23. - PubMed
    1. Reynolds MA. Modifiable risk factors in periodontitis: At the intersection of aging and disease. Periodontol 2000. 2014;64:7–19. - PubMed
    1. Soskolne WA, Klinger A. The relationship between periodontal diseases and diabetes: An overview. Ann Periodontol. 2001;6:91–8. - PubMed
    1. Taylor GW. Bidirectional interrelationships between diabetes and periodontal diseases: An epidemiologic perspective. Ann Periodontol. 2001;6:99–112. - PubMed