A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells
- PMID: 35959771
- PMCID: PMC10227723
- DOI: 10.1039/d2ay00931e
A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells
Abstract
In this paper, a 3D-printed multi-modal device was designed and fabricated to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions prepared from whole blood. Once a sample was injected into the device, NO was first detected (via amperometry) using a three-electrode, dual-opposed, electrode configuration with a platinum-black/Nafion coated gold working electrode. After in-line amperometric detection of NO, ATP was detected via a chemiluminescence reaction, with a luciferin/luciferase solution continuously pumped into an integrated mixing T and the resulting light being measured with a PMT underneath the channel. The device was optimized for mixing/reaction conditions, limits of detection (40 nM for NO and 30 nM for ATP), and sensitivity. This device was used to determine the basal (normoxic) levels of NO and ATP in red blood cells, as well as an increase in concentration of both analytes under hypoxic conditions. Finally, the effect of storing red blood cells in a commonly used storage solution was also investigated by monitoring the production of NO and ATP over a three-week storage time.
Figures






Similar articles
-
3D-Printed Microfluidic Device with In-line Amperometric Detection that Also Enables Multi-Modal Detection.Anal Methods. 2020 Apr 21;12(15):2046-2051. doi: 10.1039/d0ay00368a. Epub 2020 Mar 27. Anal Methods. 2020. PMID: 32849919 Free PMC article.
-
3D printed microfluidic devices with integrated versatile and reusable electrodes.Lab Chip. 2014 Jun 21;14(12):2023-32. doi: 10.1039/c4lc00171k. Epub 2014 Apr 25. Lab Chip. 2014. PMID: 24763966 Free PMC article.
-
PolyJet 3D-Printed Enclosed Microfluidic Channels without Photocurable Supports.Anal Chem. 2019 May 21;91(10):6910-6917. doi: 10.1021/acs.analchem.9b01302. Epub 2019 May 8. Anal Chem. 2019. PMID: 31035747 Free PMC article.
-
Endothelium-derived nitric oxide production is increased by ATP released from red blood cells incubated with hydroxyurea.Nitric Oxide. 2014 Apr 30;38:1-7. doi: 10.1016/j.niox.2014.02.003. Epub 2014 Feb 12. Nitric Oxide. 2014. PMID: 24530476
-
3D printed microfluidics for biological applications.Lab Chip. 2015;15(18):3627-37. doi: 10.1039/c5lc00685f. Lab Chip. 2015. PMID: 26237523 Review.
Cited by
-
3D printed microfluidic devices for integrated solid-phase extraction and microchip electrophoresis of preterm birth biomarkers.Anal Chim Acta. 2024 Apr 1;1296:342338. doi: 10.1016/j.aca.2024.342338. Epub 2024 Feb 5. Anal Chim Acta. 2024. PMID: 38401930 Free PMC article.
-
In-silico investigation reveals microbial metabolic biomarkers and their regulatory roles in hormone sensitive cancers.J Genet Eng Biotechnol. 2025 Sep;23(3):100549. doi: 10.1016/j.jgeb.2025.100549. Epub 2025 Aug 6. J Genet Eng Biotechnol. 2025. PMID: 40854667 Free PMC article.
-
Assessment of stored red blood cells through lab-on-a-chip technologies for precision transfusion medicine.Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2115616120. doi: 10.1073/pnas.2115616120. Epub 2023 Jul 26. Proc Natl Acad Sci U S A. 2023. PMID: 37494421 Free PMC article.
-
3D printed microfluidic devices with electrodes for electrochemical analysis.Anal Methods. 2024 Oct 24;16(41):6941-6953. doi: 10.1039/d4ay01701c. Anal Methods. 2024. PMID: 39403769 Review.
-
High-performance microchip electrophoresis separations of preterm birth biomarkers using 3D printed microfluidic devices.J Chromatogr A. 2023 Sep 13;1706:464242. doi: 10.1016/j.chroma.2023.464242. Epub 2023 Aug 1. J Chromatogr A. 2023. PMID: 37595419 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources