Effects of strontium on the morphological and photosynthetic physiological characteristics of Vicia faba seedlings

Abstract

The adaptation of plants to strontium (Sr) stress requires a more systematic understanding. In the present study, the morphological and photosynthetic physiological characteristics of Vicia faba seedlings under Sr stress (⁸⁸Sr, 0-1,000 mg·L^-1) were analyzed in solution culture. The results showed that Sr treatment decreased the biomass and root activity of V. faba seedlings significantly, but fortunately, there was almost no root necrosis. In plant morphology, the taproot length, lateral root number, plant height, branching number and internodes number of V. faba were significantly inhibited, thus the apical dominance of taproot and terminal bud was more obvious. The accumulation of Sr resulted in the decrease of leaf area, dry weight, stomatal density and stomatal aperture, while the guard cell length increased, and the specific leaf weight (SLW) increased first and then decreased. These changes in stomatal morphology may be a positive regulation to reduce water loss. In addition, V. faba increased the non-photochemical quenching (NPQ) and the activities of peroxidase (POD) and ascorbate peroxidase (APX) to protect the photosynthetic structure. Low concentration of Sr (250 mg·L^-1) promoted the photochemical efficiency and electron transfer of PSII (e.g., increased F_v/F_m, Φ_PSII, qP and ETR). However, Sr (250-1,000 mg·L^-1) inhibited the net photosynthetic rate (P_n), transpiration rate (T_r) and stomatal conductance (G_s) in leaves. In general, the P_n was affected by both stomatal and non-stomatal factors. Since Sr did not cause significant damage to the PSII function, the non-stomatal factor may be the dark reaction in photosynthesis affected, but this needs to be proved by further studies.

Keywords: Antioxidant enzymes; chlorophyll fluorescence; morphology; nuclear contamination; photosynthesis.