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Comment
. 2023 Jan;41(1):29-30.
doi: 10.1038/s41587-022-01423-x.

Engineered Cas13 variants with minimal collateral RNA targeting

No authors listed
Comment

Engineered Cas13 variants with minimal collateral RNA targeting

No authors listed. Nat Biotechnol. 2023 Jan.
No abstract available

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References

    1. Smargon, A. A., Shi, Y. J. & Yeo, G. W. RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering. Nat. Cell Biol. 22, 143–150 (2020). A Review article that presents progress in the RNA-targeting CRISPR–Cas field, including the applications of Cas13 for RNA biology. - DOI
    1. Zhang, C. et al. Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d. Cell 175, 212–223.e217 (2018). This paper reports the molecular and structural basis for Cas13d function, including both guide and target RNA recognition. - DOI
    1. Slaymaker, I. M. et al. High-resolution structure of Cas13b and biochemical characterization of RNA targeting and cleavage. Cell Rep. 26, 3741–3751.e3745 (2019). This paper reports a high-resolution structure of Cas13b and demonstrates that Cas13b can be rationally engineered to change RNA cleavage specificity. - DOI
    1. Wang, Q. et al. The CRISPR-Cas13a gene-editing system induces collateral cleavage of RNA in glioma cells. Adv. Sci. (Weinh.) 6, 1901299 (2019). This paper reports collateral effects of the CRISPR–Cas13a system in eukaryotes for the first time and demonstrates the powerful tumor-eliminating potential of this system.
    1. Ai, Y., Liang, D. & Wilusz, J. E. CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells. Nucleic Acids Res. 50, e65 (2022). This paper reports that Cas13d and Cas13b have differing extents of off-target effects and highlights the need for caution when using Cas13 effectors in eukaryotic cells. - DOI

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