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. 2022 Aug 8:14:1037-1045.
doi: 10.2147/IJWH.S357140. eCollection 2022.

Overexpression of miR-3653 is Associated with HPV Infection and Serves as a Biomarker in Patients with Cervical Cancer

Haiyan Cui  1 , Baohua Zhang  2 , Mei Ruan  3 , Chunmei Fang  4 , Ning Li  5 , Xiaoqin Sun  6 , Junmei Qi  1 , Rongrong Zuo  1 , Shuangshuang Zhang  1 , Jiansheng Rong  2
Affiliations

Overexpression of miR-3653 is Associated with HPV Infection and Serves as a Biomarker in Patients with Cervical Cancer

Haiyan Cui et al. Int J Womens Health. .

Abstract

Background: Human papillomavirus (HPV) is a major cause of cervical cancer (CC) occurrence. This study aimed to explore whether abnormal microRNA (miR)-3653 is associated with HPV infection and to investigate the clinical value of miR-3653 in the diagnosis and prognosis of CC.

Methods: Tumor tissues and adjacent non-cancerous tissues were collected from 136 patients with CC. Cervical tissues from 101 patients with uterine fibroids were collected as controls. The expression of miR-3653 was measured by quantitative real-time PCR. The ability of miR-3653 to discriminate between HPV positive (HPV+) and HPV negative (HPV-) CC patients, and to discriminate patients from controls was assessed by receiver operating characteristic analysis. Kaplan-Meier curves and Log rank tests were used to evaluate the relationship of miR-3653 with survival of CC patient. Whether miR-3653 could function as a prognostic indicator was evaluated by univariate and multivariate Cox analyses.

Results: miR-3653, highly expressed in CC tissues, was associated with HPV infection, tumor diameter, International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis in CC patients. Additionally, miR-3653 was increased in HPV+ controls, CC patients and CC cells. Moreover, miR-3653 could screen HPV+ controls, screen HPV+ patients and screen CC patients. Furthermore, miR-3653 was associated with the survival of CC patients (log-rank P < 0.001) and could serve as an independent prognostic indicator for CC patients.

Conclusion: miR-3653, increased in CC, is related to HPV infection and may serve as a diagnostic and prognostic biomarker for CC patients.

Keywords: HPV; cervical cancer; diagnosis; miR-3653; microRNA; prognosis.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Expression of miR-3653 in CC patients and adjacent non-cancerous tissues. *P < 0.05 vs adjacent non-cancerous tissues.
Figure 2
Figure 2
Expression of miR-3653 in CC patients and cell lines with different HPV infection conditions. (A) Expression of miR-3653 in controls and CC patients with different HPV infection conditions. (B) Expression of miR-3653 in CC cell lines with different HPV infection conditions. *P < 0.05 vs HPV- Controls or HaCaT; #P < 0.05 vs HPV+ Controls; &P < 0.05 vs HPV- CC or C33A.
Figure 3
Figure 3
Diagnostic value of miR-3653. (A) A ROC curve based on miR-3653 expression in discriminating between HPV+ controls HPV- controls. (B) A ROC curve based on miR-3653 expression in discriminating between HPV+ and HPV- CC patients. (C) A ROC curve based on miR-3653 to screen CC patients from controls.
Figure 4
Figure 4
High miR-3653 was associated with poor prognosis in CC patients (log-rank P < 0.001).
See this image and copyright information in PMC

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