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. 2022 Jul 28:9:930959.
doi: 10.3389/fcvm.2022.930959. eCollection 2022.

Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia

Antonio Concistrè  1 , Luigi Petramala  2 , Francesco Circosta  1 , Priscilla Romagnoli  1 , Maurizio Soldini  1 , Marco Bucci  3 , Domenico De Cesare  3 , Giuseppe Cavallaro  4 , Giorgio De Toma  4 , Francesco Cipollone  3 , Claudio Letizia  1
Affiliations

Analysis of the miRNA expression from the adipose tissue surrounding the adrenal neoplasia

Antonio Concistrè et al. Front Cardiovasc Med. .

Abstract

Background: Primary aldosteronism (PA) is characterized by several metabolic changes such as insulin resistance, metabolic syndrome, and adipose tissue (AT) inflammation. Mi(cro)RNAs (miRNAs) are a class of non-coding small RNA molecules known to be critical regulators in several cellular processes associated with AT dysfunction. The aim of this study was to evaluate the expression of some miRNAs in visceral and subcutaneous AT in patients undergoing adrenalectomy for aldosterone-secreting adrenal adenoma (APA) compared to the samples of AT obtained in patients undergoing adrenalectomy for non-functioning adrenal mass (NFA).

Methods: The quantitative expression of selected miRNA using real-time PCR was analyzed in surrounding adrenal neoplasia, peri-renal, and subcutaneous AT samples of 16 patients with adrenalectomy (11 patients with APA and 5 patients with NFA).

Results: Real-time PCR cycles for miRNA-132, miRNA-143, and miRNA-221 in fat surrounding adrenal neoplasia and in peri-adrenal AT were significantly higher in APA than in patients with NFA. Unlike patients with NFA, miRNA-132, miRNA-143, miRNA-221, and miRNA-26b were less expressed in surrounding adrenal neoplasia AT compared to subcutaneous AT in patients with APA.

Conclusion: This study, conducted on tissue expression of miRNAs, highlights the possible pathophysiological role of some miRNAs in determining the metabolic alterations in patients with PA.

Keywords: adipose tissue; adrenalectomy; aldosterone-secreting adrenal adenoma; miRNAs; primary aldosteronism.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Linear correlation between the C-reactive protein values and the expression of miR-26b (r = 0.825; p = 0.043).
Figure 2
Figure 2
Comparison between the quantitative expression of surrounding adrenal neoplasia AT miRNAs and follow-up data. BMI, body mass index; NC, neck circumference; LDL-C, LDL cholesterol; LVMI, left ventricular mass indexed for the surface body area.
Figure 3
Figure 3
Physiological and pathophysiological relationship between aldosterone production and hemodynamic, metabolic, and molecular balance. NFA, non-functional adrenal mass; APA, aldosterone-secreting adrenal adenoma; BP, blood pressure; ROS, reactive oxigene species.
See this image and copyright information in PMC

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