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. 2022 Jul 28:13:922989.
doi: 10.3389/fmicb.2022.922989. eCollection 2022.

Effect of Cultivated Pastures on Soil Bacterial Communities in the Karst Rocky Desertification Area

Shuzhen Song  1   2 , Kangning Xiong  1   2 , Yongkuan Chi  1   2 , Cheng He  1 , Jinzhong Fang  1 , Shuyu He  1
Affiliations

Effect of Cultivated Pastures on Soil Bacterial Communities in the Karst Rocky Desertification Area

Shuzhen Song et al. Front Microbiol. .

Abstract

Soil bacteria play an important role in regulating the process of vegetation restoration in karst ecosystems. However, the effects of vegetation restoration for different cultivated pastures on soil bacterial communities in the karst rocky desertification regions remain unclear. Therefore, we hypothesized that mixed pasture is the most effective for soil bacterial communities among different vegetation restorations. In this study, we systematically studied the soil properties and soil bacterial communities in four vegetation restoration modes [i.e., Dactylis glomerata pasture (DG), Lolium perenne pasture (LP), Lolium perenne + Trifolium repens mixed pasture (LT), and natural grassland (NG)] by using 16S rDNA Illumina sequencing, combined with six soil indicators and data models. We found that the vegetation restoration of cultivated pastures can improve the soil nutrient content compared with the natural grassland, especially LT treatment. LT treatment significantly increased the MBC content and Shannon index. The vegetation restoration of cultivated pastures significantly increased the relative abundance of Proteobacteria, but LT treatment significantly decreased the relative abundance of Acidobacteria. Soil pH and MBC significantly correlated with the alpha diversity of soil bacterial. Soil pH and SOC were the main factors that can affect the soil bacterial community. FAPROTAX analysis showed LT treatment significantly decreased the relative abundance of aerobic chemoheterotrophs. The results showed that the bacterial communities were highly beneficial to soil restoration in the LT treatment, and it confirmed our hypothesis. This finding provides a scientific reference for the restoration of degraded ecosystems in karst rocky desertification areas.

Keywords: Illumina sequencing; cultivated pasture; karst rocky desertification; soil bacterial community; soil properties; vegetation restoration.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Soil properties and soil microbial biomass in different vegetation restorations. Boxplots with different lowercase letters are significantly different among different sites (p < 0.05). (A) The content of SOC; (B) the content of TN; (C) the content of TP; (D) pH value; (E) the content of MBC; (F) the content of MBN. DG, Dactylis glomerata; LP, Lolium perenne; LT, Trifolium repens; NG, natural grassland; SOC, soil organic carbon; TN, total nitrogen; TP, total phosphorus; MBC, soil microbial biomass C; MBN, soil microbial biomass N.
FIGURE 2
FIGURE 2
Principal coordinates analysis of bacterial in different vegetation restorations. DG, Dactylis glomerata; LP, Lolium perenne; LT, Trifolium repens; NG, natural grassland.
FIGURE 3
FIGURE 3
Relative abundance of soil bacterial phylum in different vegetation restorations. Different lowercase letters are significantly different among different sites (p < 0.05). DG, Dactylis glomerata; LP, Lolium perenne; LT, Trifolium repens; NG, natural grassland.
FIGURE 4
FIGURE 4
RDA for the relationship between the bacterial phylum (blue arrows) and environmental factors (red arrows). SOC, soil organic carbon; Proteoba, Proteobacteria; Acidobac, Acidobacteriota; Verrucom, Verrucomicrobiota; Chlorofl, Chloroflexi; unclassi, unclassified Bacteria; Bacteroi, Bacteroidota; Gemmatim, Gemmatimonadota; Actinobc, Actinobacteriota; Myxococc, Myxococcota; Firmicut, Firmicutes.
FIGURE 5
FIGURE 5
Relative abundance of soil bacterial community for ecological function in different vegetation restorations. Different lowercase letters are significantly different among different sites (p < 0.05). DG, Dactylis glomerata; LP, Lolium perenne; LT, Trifolium repens; NG, natural grassland.
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