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. 2022 Aug 8:15:1583-1591.
doi: 10.2147/CCID.S371330. eCollection 2022.

Exosomes Derived from Secretome Human Umbilical Vein Endothelial Cells (Exo-HUVEC) Ameliorate the Photo-Aging of Skin Fibroblast

Endra Yustin Ellistasari  1 , Harijono Kariosentono  2 , Bambang Purwanto  3 , Brian Wasita  4 , Risya Cilmiaty Arief Riswiyant  5 , Eti Poncorini Pamungkasari  6 , Soetrisno Soetrisno  7
Affiliations

Exosomes Derived from Secretome Human Umbilical Vein Endothelial Cells (Exo-HUVEC) Ameliorate the Photo-Aging of Skin Fibroblast

Endra Yustin Ellistasari et al. Clin Cosmet Investig Dermatol. .

Abstract

Purpose: This is an in-vitro experimental study to analyze the effect of Exo-HUVEC on endothelial cell (CD31), cell proliferation, matrix metalloproteinase 1 (MMP-1) and collagen type 1 on irradiated fibroblast with UVB as photo-aging model.

Patients and methods: Fibroblast cultures were divided into 5 groups, namely without UVB exposure, UVB exposure 600mJ/cm2 for 80 seconds as photo-aging model, and UVB exposure +Exo-HUVEC exposure 0.1%, 0.5% and 1%. The endothelial cell was stained with a CD31 marker, MMP-1 were examined with ELISA, cell proliferation is detected using an MTT assay; meanwhile, collagen type 1 deposition and endothelial cell were measured using flowcytometry.

Results: This study found positive endothelial cell marker CD31. Significant difference was found in cell proliferation, MMP-1 and collagen type 1 level between the control group with UVB irradiation and the treatment group with Exo-HUVEC (p < 0.05).

Conclusion: Exo-HUVEC significantly increases cell proliferation and collagen type 1 level, while decrease MMP-1 levels on irradiated fibroblast; therefore, Exo-HUVEC ameliorate the photo-aging of skin fibroblast.

Keywords: Exo-HUVEC; MMP-1; cell proliferation; collagen type 1; endothelial cell; photo-aging.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

None
Graphical abstract
Figure 1
Figure 1
Morphology of fibroblast cells.
Figure 2
Figure 2
Flow cytometry result in CD31 expression (left: unstained; right: stained).
Figure 3
Figure 3
Mean MTT Assay. K1: control group without UVB irradiation, K2: Control group with UVB irradiation, P1: 0.1% Exo-HUVEC, P2: 0.5% Exo-HUVEC, P3: 1% EXO-HUVEC.
Figure 4
Figure 4
Mean MMP-1 level. K1: control group without UVB irradiation, P1: 0.1% Exo-HUVEC, P2: 0.5% Exo-HUVEC, P3: 1% Exo-HUVEC.
Figure 5
Figure 5
Flowcytometry result of collagen type 1 level. K1: control group without UVB irradiation, K2: control group with UVB irradiation, P1: 0.1% Exo-HUVEC, P2: 0.5% Exo-HUVEC, P3: 1% Exo-HUVEC.
None
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