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. 2022 Apr-Jun;26(2):185-191.
doi: 10.4103/jomfp.jomfp_9_21. Epub 2022 Jun 28.

Glucose transporter 1 expression in ameloblastoma and odontogenic keratocyst - A comparative immunohistochemical study

Sindhuri Pragallapati  1 , Ravikanth Manyam  1
Affiliations

Glucose transporter 1 expression in ameloblastoma and odontogenic keratocyst - A comparative immunohistochemical study

Sindhuri Pragallapati et al. J Oral Maxillofac Pathol. 2022 Apr-Jun.

Abstract

Introduction: Facilitative glucose transporters (GLUTs), which mediate glucose transport across the cell membrane, differ in their tissue distribution and affinity for glucose. GLUT1 is ubiquitously present and help in the basal uptake of glucose into the cells. Its expression is known to be elevated in conditions that induce hypoxia and by growth factors. GLUT1 is known to be increased in many malignant tumors to meet the metabolic requirements, but its role in odontogenic tumors is not known.

Objective: The objective of this study is to evaluate and compare the immunohistochemical expression of GLUT1 in ameloblastoma (AM) and odontogenic keratocyst (OKC).

Materials and methodology: Thirty cases each of AM and OKCs were immunohistochemically stained using anti-GLUT1 antibody according to the standard protocol. Qualitative assessment of GLUT1 expression was done under the categories of distribution, intensity and localization of staining. Quantitative assessment was done using Image J software. The results were tabulated and statistically analyzed.

Results: GLUT1 positivity was observed in 25 (83.3%) cases of OKC and 26 (86.7%) of AM cases. The majority of cells in the suprabasal layer of OKC showed positivity, whereas the equal distribution of staining was observed in the central and peripheral cells of AM.

Conclusion: GLUT1 expression in these tumors is suggestive of an increased glucose uptake and probably increased utilization of energy, which may be correlated with their aggressive behavior.

Keywords: Ameloblastoma; glucose transporters 1; odontogenic keratocyst; odontogenic tumors; remmele score.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Protocol followed for qualitative and quantitative evaluation of glucose transporters 1 immunostaining
Figure 2
Figure 2
(a) Intensely stained cells localized predominantly in the supra basal layers of odontogenic keratocyst. Cytoplasmic staining is seen in the basal layer and membrane staining is seen in suprabasal layers (×10). (b) Equal staining distribution between central and peripheral cells of follicular ameloblastoma (×40)
Figure 3
Figure 3
(a) Odontogenic keratocyst and (b) ameloblastoma lining showing focal clusters of intensely stained cells with predominant membrane staining (×40)
See this image and copyright information in PMC

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References

    1. Lienhard GE, Slot JW, James DE, Mueckler MM. How cells absorb glucose. Sci Am. 1992;266:86–91. - PubMed
    1. Augustin R. The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. 2010;62:315–33. - PubMed
    1. Carruthers A, DeZutter J, Ganguly A, Devaskar SU. Will the original glucose transporter isoform please stand up! Am J Physiol Endocrinol Metab. 2009;297:E836–48. - PMC - PubMed
    1. Parente P, Coli A, Massi G, Mangoni A, Fabrizi MM, Bigotti G. Immunohistochemical expression of the glucose transporters GLUT1 and Glut-3 in human malignant melanomas and benign melanocytic lesions. J Exp Clin Cancer Res. 2008;27:34. - PMC - PubMed
    1. Ito S, Fukusato T, Nemoto T, Sekihara H, Seyama Y, Kubota S. Coexpression of glucose transporter 1 and matrix metalloproteinase-2 in human cancers. J Natl Cancer Inst. 2002;94:1080–91. - PubMed