A CRISPR upgrade unlocks Toxoplasma gene function

Aurelie Tsee Dawson¹, Christopher J Tonkin²

Affiliations

¹ The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia.
² The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia. Electronic address: tonkin@wehi.edu.au.

PMID: 35973902
DOI: 10.1016/j.pt.2022.07.007

Comment

A CRISPR upgrade unlocks Toxoplasma gene function

Aurelie Tsee Dawson et al. Trends Parasitol. 2022 Oct.

. 2022 Oct;38(10):826-828.

doi: 10.1016/j.pt.2022.07.007. Epub 2022 Aug 13.

Authors

Aurelie Tsee Dawson¹, Christopher J Tonkin²

Affiliations

¹ The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia.
² The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia. Electronic address: tonkin@wehi.edu.au.

PMID: 35973902
DOI: 10.1016/j.pt.2022.07.007

Abstract

Forward genetic screens are invaluable in describing gene function. CRISPR has reinvigorated phenotypic screens in Toxoplasma - a model apicomplexan parasite. Two recent papers by Smith et al. and Li et al. take the next big leap in performing forward genetic screens in Toxoplasma by combining conditional gene regulation with CRISPR.

Keywords: Apicomplexa; CRISPR; Toxoplasma; auxin-inducible degron; phenotypic screening; split Cas9.

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Conflict of interest statement

Declaration of interests The authors declare no competing interests.

Comment on

Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress.
Smith TA, Lopez-Perez GS, Herneisen AL, Shortt E, Lourido S. Smith TA, et al. Nat Microbiol. 2022 Jun;7(6):868-881. doi: 10.1038/s41564-022-01104-0. Epub 2022 Apr 28. Nat Microbiol. 2022. PMID: 35484233 Free PMC article.
A splitCas9 phenotypic screen in Toxoplasma gondii identifies proteins involved in host cell egress and invasion.
Li W, Grech J, Stortz JF, Gow M, Periz J, Meissner M, Jimenez-Ruiz E. Li W, et al. Nat Microbiol. 2022 Jun;7(6):882-895. doi: 10.1038/s41564-022-01114-y. Epub 2022 May 10. Nat Microbiol. 2022. PMID: 35538310

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A CRISPR upgrade unlocks Toxoplasma gene function

Affiliations

A CRISPR upgrade unlocks Toxoplasma gene function

Authors

Affiliations

Abstract

Conflict of interest statement

Comment on

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MeSH terms

LinkOut - more resources

Full Text Sources