Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel

Abstract

Objective: The study aims to assess the effects of a 10% H₂O₂ bleaching gel with different MnO₂ concentrations on the bleaching efficacy (BE), degradation kinetics (DK) of H₂O₂, and trans-amelodentinal cytotoxicity (TC).

Materials and methods: Standardized bovine enamel/dentin disks (n = 96) were placed in artificial pulp chambers, and the bleaching gels were applied for 45 min. Thus, the following groups were established: (G1) no treatment (negative control/NC); (G2) 35% H₂O₂ (positive control/PC); (G3) 10% H₂O₂; (G4) 10% H₂O₂ + 2 mg/mL MnO₂; (G5) 10% H₂O₂ + 6 mg/mL MnO₂; and (G6) 10% H₂O₂ + 10 mg/mL MnO₂. After analyzing bleaching efficacy (ΔE₀₀ and ΔWI), the degradation kinetics of H₂O₂ and trans-amelodentinal cytotoxicity were determined (n = 8, ANOVA/Tukey; p < 0.05).

Results: G6 presented BE (ΔE₀₀ and ΔWI) statistically similar to G2, which represented conventional in-office bleaching (p = 0.6795; p > 0.9999). A significant reduction in the diffusion of H₂O₂ occurred in G3, G4, G5, and G6 compared to G2 (p < 0.0001). The highest DK of H₂O₂ occurred in G6 (p < 0.0001), which had the lowest TC in comparison with all other bleached groups (p ≤ 0.0186).

Conclusion: The addition of 10 mg/mL of MnO₂ in a 10% H₂O₂ bleaching gel potentiates the degradation of this reactive molecule, which increases the BE of the product and decreases TC.

Clinical significance: Replacing a 35% H₂O₂ gel commonly used for conventional in-office dental bleaching by a 10% H₂O₂ gel containing 10 mg/mL of MnO₂ reduces the cytotoxicity of this professional therapy, maintaining its excellent esthetic efficacy.

Keywords: Cell culture; Dental materials; Tooth bleaching; Toxicity.