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Published Erratum
. 2022 Aug 30;119(35):e2212111119.
doi: 10.1073/pnas.2212111119. Epub 2022 Aug 17.

Correction for Rodriguez-Hernandez et al., Microbiome-mediated incapacitation of interferon lambda production in the oral mucosa

No authors listed
Published Erratum

Correction for Rodriguez-Hernandez et al., Microbiome-mediated incapacitation of interferon lambda production in the oral mucosa

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 1.
Fig. 1.
IFN-λ is strongly expressed in gingival tissues, cell lines, and activates ISG expression in oral tissues. (A) IFNL-R expression was determined in fixed human gingival tissues from healthy donors by immunofluorescent staining. (B) Oral epithelial cells were stimulated with 5 μg/mL HSV60 or 50 μg/mL Poly I:C for 24 h, and IFN-λ and IFN-β levels measured in cell-free supernatants by ELISA. Data are shown as mean ± SD and statistical differences determined by two-way ANOVA with Holm–Sidak multiple comparison test (*P < 0.05; **P < 0.01; φP < 0.001). (C) TIGKs were treated with 20 ng/mL IFN-λ1 or (D) 0.25 ng/mL IFN-β for 24 h and analyzed by RNA-seq. Hierarchical clustering heatmap (based on log [RPKM] values) of the top 50 ISGs induced by IFN-λ1 or IFN-β compared to untreated cells are shown. Color intensity denotes level of gene expression. (E) Mx1gfp mice were intraperitoneally (i.p.) injected with 50 μg Poly I:C or 4 μg IFN-λ and euthanized after 36 h. GECs (EpCAM+, CD45-) and leukocytes (EpCAM-, CD45+) were analyzed by flow cytometry, and % GFP-positive cells (mean ± SD) determined by flow cytometry for each population from three mice per group. Statistical differences were determined by one-way ANOVA and Dunnett's correction (*P < 0.05; **P < 0.01). GECs isolated from healthy human donors or periodontitis patients were stimulated with 5 μg/mL HSV60 or 50 μg/mL Poly I:C for 24 h. (F) IFN-λ was measured by ELISA and (G) ISG15 expression was determined by qRT-PCR, and normalized to GADPH (2-ΔΔCT). Data are shown as mean ± SD and statistical differences determined by two-way ANOVA with Holm–Sidak multiple comparison test (**P < 0.01; ***P < 0.001).
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