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. 2022:2540:361-385.
doi: 10.1007/978-1-0716-2541-5_19.

Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy

Olympia-Ekaterini Psathaki  1   2 , Achim Paululat  3
Affiliations

Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy

Olympia-Ekaterini Psathaki et al. Methods Mol Biol. 2022.

Abstract

Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing-freeze substitution (HPF-FS) to preserve cellular structures.

Keywords: Chemical fixation; Drosophila; Freeze-substitution; High-pressure freezing; Transmission electron microscopy.

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References

    1. Hurd TR, Sanchez CG, Teixeira FK, Petzold C, Dancel-Manning K, Wang JY, Lehmann R, Liang FX (2015) Ultrastructural analysis of drosophila ovaries by electron microscopy. Methods Mol Biol 1328:151–162. https://doi.org/10.1007/978-1-4939-2851-4_11
    1. Suvarna SK, Layton C, Bancroft JD (2019) Bancroft’s theory and practice of histological techniques, 8th edn. Elsevier
    1. Darley JJ, Ezoe H (1976) Potential hazards of uranium and its compounds in electron microscopy: a brief review. J Microsc 106:85–96
    1. Tepass U, Hartenstein V (1994) Epithelium formation in the Drosophila midgut depends on the interaction of endoderm and mesoderm. Development 120:579–590
    1. McDonald KL, Sharp DJ, Rickoll W (2000) Preparation of thin sections of Drosophila for examination by transmission electron microscopy. In: Sullivan W, Ashburner M, Hawley RS (eds) Drosophila protocols. Cold Spring Harbor Laboratory Press, New York, pp 245–271

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