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. 2022 Aug;65(10-11):288-291.
doi: 10.1002/jlcr.3997. Epub 2022 Aug 23.

Simplified and accessible [18 F]F-AraG synthesis procedure for preclinical PET

Antonia A Högnäsbacka  1 , Miguel A Cortés González  1 , Christer Halldin  1 , Magnus Schou  1   2
Affiliations

Simplified and accessible [18 F]F-AraG synthesis procedure for preclinical PET

Antonia A Högnäsbacka et al. J Labelled Comp Radiopharm. 2022 Aug.

Abstract

The PET tracer [18 F]F-AraG, an arabinosyl guanine analog, has shown promise for visualizing activated T cells in multiple diseases. Herein, a practitioner's protocol is described, in which the PET tracer is prepared using minimal equipment and manual actions, making it widely accessible for preclinical applications.

Keywords: AraG; SPE purification, T cell imaging; [18F]F-AraG; nucleophilic fluorination; nucleophilic substitution; practitioner's protocol.

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Conflict of interest statement

Magnus Schou is an employee and shareholder at AstraZeneca.

Figures

FIGURE 1
FIGURE 1
[18F]F‐AraG production protocol, where blue describes the [18F]Fluoride production, trapping, and elution; black the one‐pot reaction; and red the work‐up and formulation of the product
See this image and copyright information in PMC

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