The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice

Abstract

Objective Through the preparation of the diabetic mice skin ulcer model, we investigated the effect of Mongolian medicine external ulcer powder (WYK) on the treatment of diabetic skin ulcers and the expression of angiogenesis-related factors such as vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK). Methods Thirty male clean Kunming mice were randomly divided into normal control group (group C), diabetic control group (group HC), and diabetic topical ulcer powder group (group HW). After successful modeling in the HC group and the HW group, the rats in the HW group were given external ulcer powder, which was applied to the back of the mice once a day. In addition, the rats in group C and group HC were treated with gentamicin injection external application once a day. The mice were sacrificed on the 3rd, the sixth, and the ninth day of dosing, and samples were taken. The adopted methods included protein immunoblotting (western blot) and reverse transcription-polymerase chain reaction (RT-PCR). The expression differences of angiogenesis-related factors such as VEGF and ERK in the repair process were detected. SPSS 13 software was used to analyze the results of angiogenesis-related factors VEGF and ERK. Results Comparison of VEGF and ERK Contents The serum VEGF content of mice in the HC group was significantly lower than that in the C group on days 3, 6, and 9 (p <0.05). The VEGF content in the HW group was significantly higher than that in the HC group (p <0.05). The content of ERK in serum was basically consistent with that of VEGF. The results of the western blot assay were consistent with those of the RT-PCR assay. Conclusion WYK can effectively promote the healing of skin ulcer wounds in diabetic mice, accelerate the proliferation of granulation tissue, enrich the contents of capillary blood tubes and collagen fibers, and increase the microvascular content. WYK can improve the expression level of VEGF and ERK in the serum of mice and advance the peak value of protein expression.

Keywords: diabetes foot ulcer; effect; mice; ulcer; wyk.

Figure 1. The results of western blot imaging of ERK protein in granulation tissue of mice wound on the 3rd, 6th, and 9th days of administration were obtained.

ERK: Extracellular regulated protein kinases.

Figure 2. The results of western blot imaging of VEGF protein in granulation tissue of mice wound on the 3rd, 6th, and 9th days of administration were obtained.

VEGF: Vascular endothelial growth factor.

Figure 3. ERK and VEGF mRNA detected by RT-PCR.

ERK: Extracellular regulated protein kinases; VEGF: Vascular endothelial growth factor; RT-PCR: Reverse transcription-polymerase chain reaction.