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. 2022 Aug 11:2022:3855368.
doi: 10.1155/2022/3855368. eCollection 2022.

Antitumor Activities of Aqueous Cinnamon Extract on 5637 Cell Line of Bladder Cancer through Glycolytic Pathway

Zeynab Aminzadeh  1 , Nasrin Ziamajidi  1   2 , Roghayeh Abbasalipourkabir  1 , Sajedeh Daei  1 , Sobhan Helbi  3 , Abbas Moridnia  4
Affiliations

Antitumor Activities of Aqueous Cinnamon Extract on 5637 Cell Line of Bladder Cancer through Glycolytic Pathway

Zeynab Aminzadeh et al. Int J Inflam. .

Abstract

Background: Pharmacotherapy with medicinal plants is a promising approach to treat cancer. Cinnamon is a medicinal plant whose properties have been proven in various fields of medical sciences. Among its biological activities, its antioxidant and antiviral effects can be mentioned. In this study, the antitumor effects of Cinnamon with a focus on glucose metabolism in bladder cancer carcinoma cell-line 5637 were investigated.

Methods: Aqueous extract of Cinnamon was prepared from Cinnamon bark. Bladder cancer 5637cell line were treated with different concentrations of aqueous extract of Cinnamon. MTT was used to evaluate cell viability at 24, 48, and 72 h. The concentration of 1.25, 2.50, and 5 mg/ml was used. Apoptosis was assessed with Hochest33258 staining. For evaluating of aqueous extract of Cinnamon effect on glycolysis, the gene expression of epidermal growth factor receptor 2 (ErbB2), heat shock protein transcription factor1 (HSF1), and lactate dehydrogenase A (LDHA), as well as protein levels of HSF1 and LDHA, LDH activity, glucose consumption, and lactate production, were measured.

Results: Aqueous extract of Cinnamon significantly decreased ErbB2, HSF1, and LDHA gene expression and also decreased the protein level of HSF1 and LDHA, LDH activity, glucose consumption, and lactate production dose-dependently (p < 0.05).

Conclusion: Our finding showed that the aqueous extract of Cinnamon can inhibit proliferation in 5637 cells by inhibition of glycolysis and induction of apoptosis.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of different concentrations of Cinnamon aqueous extract on 5637cell viability. 5637cell were treated with concentrations (0.16, 0.32, 0.64, 1.25, 2.50, 5, 10, and 20 mg/ml) for 24, 48, and 72 h Results are expressed as mean ± SD and each value is the average of at least three independent replicates. & denoted the significant difference in comparison with the control group in 24 h (P < 0.05), ∗ in 48 h (P < 0.05), # in 72 h. (P < 0.05) compared to the control group.
Figure 2
Figure 2
Effect of different concentrations of Cinnamon aqueous extract on 5637cell apoptosis by Hochest33258 staining. All ×40 magnification images are obtained by fluorescence microscopy.
Figure 3
Figure 3
Effect of different concentrations of Cinnamon aqueous extract on mRNA expression (fold change) of HSF1 (a), ErbB2 (b), and LDHA (c). Data are represented as the mean ± SD. Each value is the average of at least three independent replicates. (∗P < 0.05, ∗∗∗P < 0.001compared to the control group). ErbB2: human epidermal growth factor receptor2, HSF1: heat-shock transcription factor1, LDHA: lactate dehydrogenase A.
Figure 4
Figure 4
Effect of different concentrations of Cinnamon aqueous extract on 5637cell. (a) The image of protein extraction by the immunoblotting result, (b) HSF1, and (c) LDHA protein expression. Data are reported as the mean ± SD and each value is the average of at least three independent replicates. (∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001 compared to the control group). HSF1: heat-shock transcription factor1, LDHA: lactate dehydrogenase A.
Figure 5
Figure 5
Effect of different concentrations of Cinnamon aqueous extract on LDH activity in5637cells. Data are reported as the mean ± SD. All reported value is the average of at least three independent replicates. (∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001 compared to control group). LDH: lactate dehydrogenase.
Figure 6
Figure 6
Effect of different concentrations of Cinnamon aqueous extract on glucose consumption (a) and lactate production (b) in 5637 cells. Data are represented as the mean ± SD. All value is the average of at least three independent replicates. (∗∗P < 0.01 and ∗∗∗P < 0.001 compared to the control group).
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