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. 2022 Jul 31;13(3):109.
doi: 10.3390/jfb13030109.

PLGA Nanoparticles Uptake in Stem Cells from Human Exfoliated Deciduous Teeth and Oral Keratinocyte Stem Cells

Maria Tizu  1 , Ion Mărunțelu  1 , Bogdan Mihai Cristea  2 , Claudiu Nistor  3 , Nikolay Ishkitiev  4 , Zornitsa Mihaylova  5 , Rozaliya Tsikandelova  6 , Marina Miteva  4 , Ana Caruntu  7   8 , Cristina Sabliov  9 , Bogdan Calenic  1 , Ileana Constantinescu  1
Affiliations

PLGA Nanoparticles Uptake in Stem Cells from Human Exfoliated Deciduous Teeth and Oral Keratinocyte Stem Cells

Maria Tizu et al. J Funct Biomater. .

Abstract

Polymeric nanoparticles have been introduced as a delivery vehicle for active compounds in a broad range of medical applications due to their biocompatibility, stability, controlled release of active compounds, and reduced toxicity. The oral route is the most used approach for delivery of biologics to the body. The homeostasis and function of oral cavity tissues are dependent on the activity of stem cells. The present work focuses, for the first time, on the interaction between two types of polymeric nanoparticles, poly (lactic-co-glycolic acid) or PLGA and PLGA/chitosan, and two stem cell populations, oral keratinocyte stem cells (OKSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). The main results show that statistical significance was observed in OKSCs uptake when compared with normal keratinocytes and transit amplifying cells after 24 h of incubation with 5 and 10 µg/mL PLGA/chitosan. The CD117+ SHED subpopulation incorporated more PLGA/chitosan nanoparticles than nonseparated SHED. The uptake for PLGA/chitosan particles was better than for PLGA particles with longer incubation times, yielding better results in both cell types. The present results demonstrate that nanoparticle uptake depends on stem cell type, incubation time, particle concentration, and surface properties.

Keywords: PLGA nanoparticles; human exfoliated deciduous teeth; oral keratinocyte stem cells.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
TEM pictures of (A) PLGA and (B) PLGA/Chi nanoparticles.
Figure 2
Figure 2
Immunofluorescence: oral keratinocyte stem cells uptake of PLGA/chitosan nanoparticles following 24 h exposure (blue DAPI staining was used for nuclei, while NPs are labelled with FITC and appear in green): (A) ×20 magnification; (B) ×40 magnification; (C) 3D model; (D) NPs outside cell membrane; nanoparticle concentration—5 µg/mL.
Figure 3
Figure 3
Uptake of polymeric nanoparticles by different oral keratinocyte cells at various NPs concentrations and different time points. (A) PLGA/chitosan uptake by normal keratinocytes (NKs), transit amplifying cells (TA), and oral keratinocyte stem cells (OKSCs); (B) PLGA uptake by normal keratinocytes (NKs), transit amplifying cells (TAs), and oral keratinocyte stem cells (OKSCs). Statistical significance was set at * p < 0.05 ANOVA; n = 5 independent experiments.
Figure 4
Figure 4
Immunofluorescence: SHED uptake of PLGA and PLGA/chitosan nanoparticles following 24 h exposure (blue DAPI staining was used for nuclei, while NPs are labeled with FITC and appear in green). (A) Normal SHED PLGA uptake; (B) CD117+ SHED PLGA uptake; (C) normal SHED PLGA/chitosan uptake; (D) CD117+ SHED PLGA/chitosan uptake. Magnification ×20; nanoparticle concentration 5 µg/mL.
Figure 5
Figure 5
Uptake of polymeric nanoparticles by normal and CD117+ SHEDs at various NP concentrations and different time points. (A) PLGA uptake by normal and CD117+ SHEDs; (B) PLGA/chitosan uptake by normal and CD117+ SHEDs. Statistical significance was set at * p < 0.05 and ** p < 0.01 ANOVA; n = 5 independent experiments.
See this image and copyright information in PMC

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