Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec;33(8):1190-1197.
doi: 10.1002/pca.3169. Epub 2022 Aug 23.

Alternative method for microenzymatic inhibition activities monitoring from Baccharis trimera extracts by CZE-UV

Guilherme P Campos  1 , Nerilson M Lima  1 , Juliana S Campos  2 , Gustavo A Micke  2 , Marcone A L de Oliveira  1
Affiliations

Alternative method for microenzymatic inhibition activities monitoring from Baccharis trimera extracts by CZE-UV

Guilherme P Campos et al. Phytochem Anal. 2022 Dec.

Abstract

Introduction: Capillary zone electrophoresis with direct UV detection (CZE-UV) was used to investigate the hypothesis about the extract of Baccharis trimera enzymatic activities as an analytical approach to monitoring the phenomenon.

Objective: The aim of this work was to investigate enzymatic bioactivities of the hydroalcoholic and infusion extracts of B. trimera through screening evaluation of the inhibition of the enzymes acetylcholinesterase (AChE) and α-glycosidase (α-GLY).

Method: An alternative approach using CZE-UV to hydroalcoholic and infusion extracts of B. trimera monitoring was applied to evaluate the inhibition ability of the enzymes AChE and α-GLY. The result of the reaction of acetylthiocholine (AThCh) with AChE was thiocholine (TCh) and acetic acid, and from the amount of TCh generated, the AChE inhibition was calculated. For the inhibition study of the two enzymes, the reactions of the extracts were optimised to be performed in situ, inside the capillary column, and the introduction of the solutions was performed through ordered sequential plug injections.

Results: Samples extracted with 70% ethanol presented 7.80% inhibition for AChE and 0.51% for α-GLY, while samples extracted with 96% ethanol resulted in 6.89% inhibition for AChE and no inhibition activity for α-GLY.

Conclusion: In the present work, the potentialities of CZE-UV for the study of hydroalcoholic and infusion extracts of B. trimera were demonstrated. The experimental results were useful for the calculation of the percentage of the inhibition activities of the AChE and α-GLY enzymes.

Keywords: Baccharis trimera; acetylcholinesterase; capillary electrophoresis; enzymatic inhibition; α-glycosidase.

PubMed Disclaimer

References

REFERENCES

    1. Karam TK, Dalposso LM, Casa DM, De Freitas GBL. Carqueja (Baccharis trimera): utilização terapêutica e biossíntese. Rev Bras Pl Med. 2013;15(2):280-286. doi:10.1590/S1516-05722013000200017
    1. Borella JC, Duarte DP, Novaretti AAG, et al. Variabilidade sazonal do teor de saponinas de Baccharis trimera (less.) DC (Carqueja) e isolamento de flavona. Rev Bras Farm. 2006;16(4):557-561. doi:10.1590/S0102-695X2006000400020
    1. De Azeredo FS. Validação de técnica analítica em cromatografia em camada delgada comparativa Para identificação de fármacos anorexígenos sintéticos em produtos fitoterápicos. Rev eletrônica Farm. 2004;1(2).
    1. De Oliveira CB, Comunello LN, Lunardelli A, et al. Phenolic enriched extract of Baccharis trimera presents anti-inflammatory and antioxidant activities. Molecules. 2012;17(1):1113-1123. doi:10.3390/molecules17011113
    1. Pádua BC, Silva LD, Júnior JVR, et al. Antioxidant properties of Baccharis trimera in the neutrophils of fisher rats. J Ethnopharmacol. 2010;129(3):381-386. doi:10.1016/j.jep.2010.04.018

LinkOut - more resources