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. 2023 Mar;30(3):865-872.
doi: 10.1007/s43032-022-01017-7. Epub 2022 Aug 23.

Relationship Between Number of Oocytes Retrieved and Embryo Euploidy Rate in Controlled Ovarian Stimulation Cycles

Jonathan D Buerger  1 , Jitesh Datla  2 , Shahab Minassian  3   4 , Sarah Dreibelbis  3 , Michael J Glassner  4 , John J Orris  4 , Nicolle Clements  2 , Alyssa Sheffy  4 , Sharon H Anderson  4
Affiliations

Relationship Between Number of Oocytes Retrieved and Embryo Euploidy Rate in Controlled Ovarian Stimulation Cycles

Jonathan D Buerger et al. Reprod Sci. 2023 Mar.

Abstract

This cohort study is aimed to determine if higher number of oocytes retrieved affects the rate of euploidy in the embryos of women undergoing controlled ovarian stimulation (COS) for in vitro fertilization (IVF) with preimplantation genetic testing for aneuploidy (PGT-A). A negative trend between the number of oocytes retrieved and embryo euploidy rate was observed using Visual Analytics software, especially when a higher number of oocytes were retrieved. After regression analysis, patient age was the only variable found to have a statistically significant negative effect (p < 0.0001) on euploidy rate in all regression models. Number of oocytes retrieved was not found to have a statistically significant effect on euploidy rate when analyzed per number of biopsied blastocysts (p = 0.5356), per number of oocytes retrieved (p = 0.1025), and per number of fertilized oocytes (p = 0.7241). The parameter estimates in the linear regression models were negative for number of oocytes retrieved. This study shows a statistically significant effect between patient age and embryo euploidy rate, which is already known. There is some evidence to suggest that higher number of oocytes retrieved may negatively impact the number of euploid embryos per number of oocytes retrieved based on the visual analytic graphs, p value approaching significance, and the negative parameter estimates in the regression models.

Keywords: Controlled ovarian stimulation; Euploid embryos; In vitro fertilization; Preimplantation genetic testing for aneuploidy; Retrieved oocytes.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Interactive Tableau software used to graph number of oocytes retrieved versus percent of euploid embryos with darker shades of blue indicating higher percentage of good quality embryos
Fig. 2
Fig. 2
Visual representation of mean percentages of euploid embryos per number of biopsies blastocytes in 3 age group categories
Fig. 3
Fig. 3
One-way ANOVA analysis results comparing mean percentages of euploid embryos per number of biopsies blastocysts in 3 age group categories
Fig. 4
Fig. 4
Visual representation of mean percentages of euploid embryos per number of biopsies blastocytes at 3 different PGT testing lab sites
Fig. 5
Fig. 5
One-way ANOVA analysis results comparing mean percentages of euploid embryos per number of biopsies blastocysts at 3 PGT testing labs
Fig. 6
Fig. 6
a Visual representation of mean percentages of euploid embryos per number of biopsied blastocysts in low AMH group (less than 1) and high AMH group (greater than and equal to 1). b T test comparing mean percentages of euploid embryos per number of biopsies blastocysts in low AMH group (less than 1) and high AMH group (greater than and equal to 1)
Fig. 7
Fig. 7
a Linear regression analysis using number of euploid embryos as outcome variable and number of biopsied blastocytes as dependent variable with independent variables of patient age, AMH level, PGT testing lab, and number of oocytes retrieved. b Linear regression analysis using number of euploid embryos as outcome variable and number of oocytes retrieved as dependent variable with independent variables of patient age, AMH level, PGT-A testing lab, and number of oocytes retrieved. c Linear regression analysis using number of euploid embryos as outcome variable and number of fertilized oocytes as dependent variable with independent variables of patient age, AMH level, PGT-A testing lab, and number of oocytes retrieved
Fig. 7
Fig. 7
a Linear regression analysis using number of euploid embryos as outcome variable and number of biopsied blastocytes as dependent variable with independent variables of patient age, AMH level, PGT testing lab, and number of oocytes retrieved. b Linear regression analysis using number of euploid embryos as outcome variable and number of oocytes retrieved as dependent variable with independent variables of patient age, AMH level, PGT-A testing lab, and number of oocytes retrieved. c Linear regression analysis using number of euploid embryos as outcome variable and number of fertilized oocytes as dependent variable with independent variables of patient age, AMH level, PGT-A testing lab, and number of oocytes retrieved
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