Case Reports

. 2022 Oct;41(10):1269-1273.

doi: 10.1007/s10096-022-04484-7. Epub 2022 Aug 24.

Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion

Margaux Garzaro^{1

2}, Lin-Pierre Zhao³, Nathalie De Castro⁴, Séverine Mercier-Delarue⁵, Francois Camelena^{5

6}, Sabine Pereyre⁷, Marie Gardette⁷, Béatrice Berçot^{5

6}, Marion Malphettes³, Cécile Bébéar⁷, Jean-David Bouaziz^{8

9}, Jérôme Le Goff^{5

10}, Lionel Galicier³, Maud Salmona^{11

12

13}

Affiliations

¹ Department of Clinical Immunopathology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, 1, Avenue Claude Vellefaux, 75010, Paris, France. margaux.garzaro@aphp.fr.
² Université de Paris, Paris, France. margaux.garzaro@aphp.fr.
³ Department of Clinical Immunopathology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, 1, Avenue Claude Vellefaux, 75010, Paris, France.
⁴ Department of Infectious Diseases, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
⁵ Department of Microbiology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
⁶ Bacteriology Department, Associated Laboratory of the French National Center for Bacterial Sexually Transmitted Infections, University of Paris, IAME, INSERM 1137, Paris, France.
⁷ Bacteriology Department, French National Center for Bacterial Sexually Transmitted Infections, CHU Bordeaux, and USC, University Bordeaux, 3671, Bordeaux, EA, France.
⁸ Université de Paris, Paris, France.
⁹ Department of Dermatology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
¹⁰ Team Insight, Université de Paris, INSERM U976, Paris, France.
¹¹ Department of Microbiology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France. maud.salmona@aphp.fr.
¹² Team Insight, Université de Paris, INSERM U976, Paris, France. maud.salmona@aphp.fr.
¹³ Department of Virology, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France. maud.salmona@aphp.fr.

PMID: 36001207
DOI: 10.1007/s10096-022-04484-7

Case Reports

Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion

Margaux Garzaro et al. Eur J Clin Microbiol Infect Dis. 2022 Oct.

. 2022 Oct;41(10):1269-1273.

doi: 10.1007/s10096-022-04484-7. Epub 2022 Aug 24.

Authors

Affiliations

¹ Department of Clinical Immunopathology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, 1, Avenue Claude Vellefaux, 75010, Paris, France. margaux.garzaro@aphp.fr.
² Université de Paris, Paris, France. margaux.garzaro@aphp.fr.
³ Department of Clinical Immunopathology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, 1, Avenue Claude Vellefaux, 75010, Paris, France.
⁴ Department of Infectious Diseases, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
⁵ Department of Microbiology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
⁶ Bacteriology Department, Associated Laboratory of the French National Center for Bacterial Sexually Transmitted Infections, University of Paris, IAME, INSERM 1137, Paris, France.
⁷ Bacteriology Department, French National Center for Bacterial Sexually Transmitted Infections, CHU Bordeaux, and USC, University Bordeaux, 3671, Bordeaux, EA, France.
⁸ Université de Paris, Paris, France.
⁹ Department of Dermatology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France.
¹⁰ Team Insight, Université de Paris, INSERM U976, Paris, France.
¹¹ Department of Microbiology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France. maud.salmona@aphp.fr.
¹² Team Insight, Université de Paris, INSERM U976, Paris, France. maud.salmona@aphp.fr.
¹³ Department of Virology, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France. maud.salmona@aphp.fr.

PMID: 36001207
DOI: 10.1007/s10096-022-04484-7

Abstract

A 45-year-old female patient receiving rituximab for B cell non-Hodgkin follicular lymphoma presented unexplained recurrent fever, abdominal discomfort, and pollakiuria. We performed shotgun metagenomic sequencing from peri-kidney collection that identified a co-infection with Mycoplasma hominis and Ureaplasma urealyticum. The patient recovered with sequelae after appropriate antibiotic treatment was given.

Keywords: B cell aplasia; Metagenomic next-generation sequencing; Mycoplasma hominis.

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References

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Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion

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Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion

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