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. 2022 Aug 17;12(8):759.
doi: 10.3390/metabo12080759.

Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics

Moyong Xue  1   2   3 , Ting Yao  4 , Min Xue  1 , Frédéric Francis  2 , Yuchang Qin  3 , Ming Jia  1 , Junguo Li  1 , Xu Gu  1
Affiliations

Mechanism Analysis of Metabolic Fatty Liver on Largemouth Bass (Micropterus salmoides) Based on Integrated Lipidomics and Proteomics

Moyong Xue et al. Metabolites. .

Abstract

Metabolic fatty liver disease caused by high-starch diet restricted the intensive and sustainable development of carnivorous fish such as largemouth bass. In this study, the combination liver proteomic and lipidomic approach was employed to investigate the key signaling pathways and identify the critical biomarkers of fatty liver in largemouth bass. Joint analysis of the correlated differential proteins and lipids revealed nine common metabolic pathways; it was determined that FABP1 were significantly up-regulated in terms of transporting more triglycerides into the liver, while ABCA1 and VDAC1 proteins were significantly down-regulated in terms of preventing the transport of lipids and cholesterol out of the liver, leading to triglyceride accumulation in hepatocyte, eventually resulting in metabolic fatty liver disease. The results indicate that FABP1, ABCA1 and VDAC1 could be potential biomarkers for treating metabolic fatty liver disease of largemouth bass.

Keywords: integrated analysis; largemouth bass; lipidomics; metabolic liver disease; proteomics.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Liver histopathology, enlarged lipid droplets (marked with red boxes) were clearly observed in MLD group. (MLD: metabolic liver disease).
Figure 2
Figure 2
Significantly different expressions between MLD and Normal groups. (A) Protein significantly different expression between MLD and Normal groups (red means up-regulated, blue means down-regulated). (B,C) Differentially expressed proteins in GO functional classification (B: up; C: down). (D) The subcellular localization of differentially expressed proteins. (E,F) KEGG enrichment pathways of different expressed proteins (E: up-regulated; F: down-regulated).
Figure 3
Figure 3
Major lipid in the liver of largemouth bass (Mean ± SEM, n = 8). (A) PC: phosphatidylcholine. (B) TG: triacylglycerol. (C) PE: phosphatidylethanolamine. (D) LPC: lysophosphatidyl choline. (E) Cer: ceramide. (F) SM: sphingomyelin. (G) LPE: lysophosphatidylethanolamine. (H) DG: diacylglycerol. (I) FFA: free fatty acid. (J) PS: phosphatidylserine. (K) CAR: acyl carnitine. (L) CE: cholesteryl ester. (M) PI: phosphatidylinositol. (N) PG: phosphatidyl glycerol. (O) LPO: lipid peroxidation.
Figure 4
Figure 4
The lipidomics analysis of largemouth bass liver. (A) Principal component analysis (PCA) score plots. (B) OPLS-DA analysis (red points and green points represent VIP ≥ 1 and VIP ≤ 1, respectively) and (C) heatmap. (Mean ± SEM, n = 8). (D) Heatmap of all lipids and proteins (red represents high correlation coefficient; green represents low correlation coefficient). (E) Heatmap of correlation coefficient of differential lipids and differential proteins (red represents a positive correlation between proteins and lipids and green represents a negative correlation). Each row and column represents a protein and a metabolite, respectively.
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References

    1. Sun C.F., Li J., Dong J.J., Niu Y.C., Hu J., Lian J.M., Li W.H., Li J., Tian Y.Y., Shi Q., et al. Chromosome-level genome assembly for the largemouth bass Micropterus salmoides provides insights into adaptation to fresh and brackish water. Mol. Ecol. Resour. 2020;21:301–315. doi: 10.1111/1755-0998.13256. - DOI - PubMed
    1. Li X.Y., Zheng S.X., Ma X.K., Cheng K.M., Wu G.Y. Effects of dietary protein and lipid levels on the growth performance, feed utilization, and liver histology of largemouth bass (Micropterus salmoides) Amino Acids. 2020;52:1043–1061. doi: 10.1007/s00726-020-02874-9. - DOI - PubMed
    1. Zhou Y., Liu C. Largemouth bass Micropterus salmoides production in China. In: Tidwell J.H., Coyle S.D., Bright L.A., editors. Largemouth Bass Aquaculture. 5M Publishing Ltd., Benchmark House; Sheffield, UK: 2019.
    1. Bai J., Li S. Development of Largemouth Bass (Micropterus salmoides) Culture. John Wiley & Sons, Ltd.; Hoboken, NJ, USA: 2018.
    1. Yu H.H., Liang X.F., Chen P., Wu X.F., Zheng Y.H., Luo L., Qin Y.C., Long X.C., Xue M. Dietary supplementation of Grobiotic®-A increases short-term inflammatory responses and improves long-term growth performance and liver health in largemouth bass (Micropterus salmoides) Aquaculture. 2018;500:327–337. doi: 10.1016/j.aquaculture.2018.10.033. - DOI