Erratum for

• Stem Cell-Laden Coaxially Electrospun Fibrous Scaffold for Regenerative Engineering Applications.

  Nagiah N, Franchi F, Peterson K, Rodriguez-Porcel M. Nagiah N, et al. Curr Protoc. 2021 Jan;1(1):e13. doi: 10.1002/cpz1.13. Curr Protoc. 2021. PMID: 33484477 Free PMC article.
• Growth and Maintenance of Escherichia coli Laboratory Strains.

  Tuttle AR, Trahan ND, Son MS. Tuttle AR, et al. Curr Protoc. 2021 Jan;1(1):e20. doi: 10.1002/cpz1.20. Curr Protoc. 2021. PMID: 33484484 Free PMC article.
• Visualization and Documentation of Capsule and Melanin Production in Cryptococcus neoformans.

  Nichols CB. Nichols CB. Curr Protoc. 2021 Jan;1(1):e27. doi: 10.1002/cpz1.27. Curr Protoc. 2021. PMID: 33484487 Free PMC article.
• Derivation of Mesenchymal Stromal Cells from Ovine Umbilical Cord Wharton's Jelly.

  Carreras-Sánchez I, López-Fernández A, Rojas-Márquez R, Vélez R, Aguirre M, Vives J. Carreras-Sánchez I, et al. Curr Protoc. 2021 Jan;1(1):e18. doi: 10.1002/cpz1.18. Curr Protoc. 2021. PMID: 33484488
• Observation of Z-DNA Structure via the Synthesis of Oligonucleotide DNA Containing 8-Trifluoromethyl-2-Deoxyguanosine.

  Bao HL, Xu Y. Bao HL, et al. Curr Protoc. 2021 Jan;1(1):e28. doi: 10.1002/cpz1.28. Curr Protoc. 2021. PMID: 33484490
• Differentiation of Brain Pericyte-Like Cells from Human Pluripotent Stem Cell-Derived Neural Crest.

  Gastfriend BD, Stebbins MJ, Du F, Shusta EV, Palecek SP. Gastfriend BD, et al. Curr Protoc. 2021 Jan;1(1):e21. doi: 10.1002/cpz1.21. Curr Protoc. 2021. PMID: 33484491 Free PMC article.
• Paraoxonase-1 (PON1) Status Analysis Using Non-Organophosphate Substrates.

  Marsillach J, Richter RJ, Costa LG, Furlong CE. Marsillach J, et al. Curr Protoc. 2021 Jan;1(1):e25. doi: 10.1002/cpz1.25. Curr Protoc. 2021. PMID: 33484495 Free PMC article.
• Chemical Synthesis of a Locked Nucleic Acid-Substituted Dinucleotide Cap Analog.

  Shanmugasundaram M, Senthilvelan A, Kore AR. Shanmugasundaram M, et al. Curr Protoc. 2021 Jan;1(1):e22. doi: 10.1002/cpz1.22. Curr Protoc. 2021. PMID: 33484497
• N-Terminal Protein Labeling with N-Hydroxysuccinimide Esters and Microscale Thermophoresis Measurements of Protein-Protein Interactions Using Labeled Protein.

  Jiang H, Cole PA. Jiang H, et al. Curr Protoc. 2021 Jan;1(1):e14. doi: 10.1002/cpz1.14. Curr Protoc. 2021. PMID: 33484499 Free PMC article.
• Fluorescence Polarization-Based Measurement of Protein-Ligand Interaction in Fungal Cell Lysates.

  LeBlanc EV, Shekhar-Guturja T, Whitesell L, Cowen LE. LeBlanc EV, et al. Curr Protoc. 2021 Jan;1(1):e17. doi: 10.1002/cpz1.17. Curr Protoc. 2021. PMID: 33484500 Free PMC article.
• Targeted Genetic Changes in Candida albicans Using Transient CRISPR-Cas9 Expression.

  Huang MY, Cravener MC, Mitchell AP. Huang MY, et al. Curr Protoc. 2021 Jan;1(1):e19. doi: 10.1002/cpz1.19. Curr Protoc. 2021. PMID: 33491919 Free PMC article.
• Laboratory Maintenance and Culture of Pseudogymnoascus destructans, the Fungus That Causes Bat White-Nose Syndrome.

  Blehert DS, Lorch JM. Blehert DS, et al. Curr Protoc. 2021 Jan;1(1):e23. doi: 10.1002/cpz1.23. Curr Protoc. 2021. PMID: 33497534
• Methods for Rapid Protein Depletion in C. elegans using Auxin-Inducible Degradation.

  Divekar NS, Horton HE, Wignall SM. Divekar NS, et al. Curr Protoc. 2021 Feb;1(2):e16. doi: 10.1002/cpz1.16. Curr Protoc. 2021. PMID: 33523606 Free PMC article.
• Quantitative Proteomic Analysis of the Senescence-Associated Secretory Phenotype by Data-Independent Acquisition.

  Neri F, Basisty N, Desprez PY, Campisi J, Schilling B. Neri F, et al. Curr Protoc. 2021 Feb;1(2):e32. doi: 10.1002/cpz1.32. Curr Protoc. 2021. PMID: 33524224 Free PMC article.
• Cap 1 Messenger RNA Synthesis with Co-transcriptional CleanCap^® Analog by In Vitro Transcription.

  Henderson JM, Ujita A, Hill E, Yousif-Rosales S, Smith C, Ko N, McReynolds T, Cabral CR, Escamilla-Powers JR, Houston ME. Henderson JM, et al. Curr Protoc. 2021 Feb;1(2):e39. doi: 10.1002/cpz1.39. Curr Protoc. 2021. PMID: 33524237
• Fully Automated Ancestral Sequence Reconstruction using FireProt^ASR.

  Khan RT, Musil M, Stourac J, Damborsky J, Bednar D. Khan RT, et al. Curr Protoc. 2021 Feb;1(2):e30. doi: 10.1002/cpz1.30. Curr Protoc. 2021. PMID: 33524240
• Expeditious Extraction of Histones from Limited Cells or Tissue Samples and Quantitative Top-Down Proteomic Analysis.

  Holt MV, Wang T, Young NL. Holt MV, et al. Curr Protoc. 2021 Feb;1(2):e26. doi: 10.1002/cpz1.26. Curr Protoc. 2021. PMID: 33534192 Free PMC article.
• Generation of Neurosphere-Derived Organoid-Like-Aggregates (NEDAS) from Neural Stem Cells.

  Watanabe F, Schoeffler A, Fair SR, Hester ME, Fedorko J, Imitola J. Watanabe F, et al. Curr Protoc. 2021 Feb;1(2):e15. doi: 10.1002/cpz1.15. Curr Protoc. 2021. PMID: 33534198
• Native Isolation of 3×HA-Tagged Protein Complexes to Characterize Protein-Protein Interactions.

  Lim J, Iftner T, Simon C. Lim J, et al. Curr Protoc. 2021 Feb;1(2):e29. doi: 10.1002/cpz1.29. Curr Protoc. 2021. PMID: 33539682
• Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents.

  Willis RA, Ramachandiran V, Shires JC, Bai G, Jeter K, Bell DL, Han L, Kazarian T, Ugwu KC, Laur O, Contreras-Alcantara S, Long DL, Altman JD. Willis RA, et al. Curr Protoc. 2021 Feb;1(2):e36. doi: 10.1002/cpz1.36. Curr Protoc. 2021. PMID: 33539685 Free PMC article.
• Laboratory Maintenance and Culturing of the Nematode-Trapping Fungus Arthrobotrys oligospora.

  Lin HC, Hsueh YP. Lin HC, et al. Curr Protoc. 2021 Feb;1(2):e41. doi: 10.1002/cpz1.41. Curr Protoc. 2021. PMID: 33544447
• Using Monochlorobimane to Visualize Glutathione Utilization in the Developing Zebrafish (Danio rerio) Embryo.

  Rastogi A, Timme-Laragy AR. Rastogi A, et al. Curr Protoc. 2021 Feb;1(2):e124. doi: 10.1002/cpz1.24. Curr Protoc. 2021. PMID: 33555621 Free PMC article.
• Immunopurification of Mitochondria from Arabidopsis.

  Luo L, He Y, Li J, Yu H. Luo L, et al. Curr Protoc. 2021 Feb;1(2):e34. doi: 10.1002/cpz1.34. Curr Protoc. 2021. PMID: 33555656
• Introducing Selenocysteine into Recombinant Proteins in Escherichia coli.

  Chung CZ, Miller C, Söll D, Krahn N. Chung CZ, et al. Curr Protoc. 2021 Feb;1(2):e54. doi: 10.1002/cpz1.54. Curr Protoc. 2021. PMID: 33566458 Free PMC article.
• Combined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus.

  Helbling JC, Kinouchi K, Trifilieff P, Sassone-Corsi P, Moisan MP. Helbling JC, et al. Curr Protoc. 2021 Feb;1(2):e33. doi: 10.1002/cpz1.33. Curr Protoc. 2021. PMID: 33566459
• Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction.

  Hoeeg C, Ringgaard L, Christensen E, Follin B, Bentsen S, Ripa RS, Kjaer A. Hoeeg C, et al. Curr Protoc. 2021 Feb;1(2):e40. doi: 10.1002/cpz1.40. Curr Protoc. 2021. PMID: 33570836
• Using Galaxy to Perform Large-Scale Interactive Data Analyses-An Update.

  Ostrovsky A, Hillman-Jackson J, Bouvier D, Clements D, Afgan E, Blankenberg D, Schatz MC, Nekrutenko A, Taylor J, Team TG, Lariviere D. Ostrovsky A, et al. Curr Protoc. 2021 Feb;1(2):e31. doi: 10.1002/cpz1.31. Curr Protoc. 2021. PMID: 33583104
• Human Plasma In-Cell Western Assays-An In vitro Predictor for In vivo Pharmacology in Oncology Drug Discovery.

  Zhang YW, Gallagher K, Angelis D, Rominger D, Scherle P, Vaddi K. Zhang YW, et al. Curr Protoc. 2021 Feb;1(2):e51. doi: 10.1002/cpz1.51. Curr Protoc. 2021. PMID: 33587334
• Traceless Staudinger Ligation for Bioconjugation of RNA.

  Kitoun C, Fonvielle M, Arthur M, Etheve-Quelquejeu M, Iannazzo L. Kitoun C, et al. Curr Protoc. 2021 Feb;1(2):e42. doi: 10.1002/cpz1.42. Curr Protoc. 2021. PMID: 33591622
• Testing Graphical Causal Models Using the R Package "dagitty".

  Ankan A, Wortel IMN, Textor J. Ankan A, et al. Curr Protoc. 2021 Feb;1(2):e45. doi: 10.1002/cpz1.45. Curr Protoc. 2021. PMID: 33592130
• In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum.

  Edmondson DG, Norris SJ. Edmondson DG, et al. Curr Protoc. 2021 Feb;1(2):e44. doi: 10.1002/cpz1.44. Curr Protoc. 2021. PMID: 33599121 Free PMC article.
• Cantharidin-Induced Skin Blister as an In Vivo Model of Inflammation.

  Dawson J, Vogelsanger M. Dawson J, et al. Curr Protoc. 2021 Feb;1(2):e49. doi: 10.1002/cpz1.49. Curr Protoc. 2021. PMID: 33600079
• Assembly and Exploration of a Single Cell Atlas of the Drosophila Larval Ventral Cord. Identification of Rare Cell Types.

  Vicidomini R, Nguyen TH, Choudhury SD, Brody T, Serpe M. Vicidomini R, et al. Curr Protoc. 2021 Feb;1(2):e37. doi: 10.1002/cpz1.37. Curr Protoc. 2021. PMID: 33600085 Free PMC article.
• A Refined Protocol for Coronary Artery Ligation in the Neonatal Mouse.

  De Villiers C, Riley PR. De Villiers C, et al. Curr Protoc. 2021 Feb;1(2):e66. doi: 10.1002/cpz1.66. Curr Protoc. 2021. PMID: 33617028
• Genomic Epidemiology Analysis of Infectious Disease Outbreaks Using TransPhylo.

  Didelot X, Kendall M, Xu Y, White PJ, McCarthy N. Didelot X, et al. Curr Protoc. 2021 Feb;1(2):e60. doi: 10.1002/cpz1.60. Curr Protoc. 2021. PMID: 33617114 Free PMC article.
• Single-Cell RNA Sequencing Analysis of the Drosophila Larval Ventral Cord.

  Nguyen TH, Vicidomini R, Choudhury SD, Coon SL, Iben J, Brody T, Serpe M. Nguyen TH, et al. Curr Protoc. 2021 Feb;1(2):e38. doi: 10.1002/cpz1.38. Curr Protoc. 2021. PMID: 33620770 Free PMC article.
• Generating Mice with Diverse Microbial Experience.

  Pierson M, Merley A, Hamilton SE. Pierson M, et al. Curr Protoc. 2021 Feb;1(2):e53. doi: 10.1002/cpz1.53. Curr Protoc. 2021. PMID: 33621444 Free PMC article.
• In Vivo Phage Display as a Biomarker Discovery Tool for the Complex Neural Injury Microenvironment.

  Martinez BI, Stabenfeldt SE. Martinez BI, et al. Curr Protoc. 2021 Feb;1(2):e67. doi: 10.1002/cpz1.67. Curr Protoc. 2021. PMID: 33625787
• Generation and Use of Recombinant Galectins.

  Wu SC, Paul A, Ho A, Patel KR, Allen JWL, Verkerke H, Arthur CM, Stowell SR. Wu SC, et al. Curr Protoc. 2021 Mar;1(3):e63. doi: 10.1002/cpz1.63. Curr Protoc. 2021. PMID: 33656274
• PIONEER: Pipeline for Generating High-Quality Spectral Libraries for DIA-MS Data.

  Manda SS, Noor Z, Hains PG, Zhong Q. Manda SS, et al. Curr Protoc. 2021 Mar;1(3):e69. doi: 10.1002/cpz1.69. Curr Protoc. 2021. PMID: 33656278
• DIAMOND+MEGAN: Fast and Easy Taxonomic and Functional Analysis of Short and Long Microbiome Sequences.

  Bağcı C, Patz S, Huson DH. Bağcı C, et al. Curr Protoc. 2021 Mar;1(3):e59. doi: 10.1002/cpz1.59. Curr Protoc. 2021. PMID: 33656283
• Automated Laboratory Growth Assessment and Maintenance of Azotobacter vinelandii.

  Carruthers BM, Garcia AK, Rivier A, Kacar B. Carruthers BM, et al. Curr Protoc. 2021 Mar;1(3):e57. doi: 10.1002/cpz1.57. Curr Protoc. 2021. PMID: 33656286
• An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat.

  Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA. Hayta S, et al. Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58. Curr Protoc. 2021. PMID: 33656289
• Murine Models for Staphylococcal Infection.

  Klopfenstein N, Cassat JE, Monteith A, Miller A, Drury S, Skaar E, Serezani CH. Klopfenstein N, et al. Curr Protoc. 2021 Mar;1(3):e52. doi: 10.1002/cpz1.52. Curr Protoc. 2021. PMID: 33656290 Free PMC article.
• Chemical Synthesis of Circular RNAs with Phosphoramidate Linkages for Rolling-Circle Translation.

  Nakamoto K, Abe H. Nakamoto K, et al. Curr Protoc. 2021 Mar;1(3):e43. doi: 10.1002/cpz1.43. Curr Protoc. 2021. PMID: 33657267
• Ultrafast Sodium Imaging of the Axon Initial Segment of Neurons in Mouse Brain Slices.

  Blömer LA, Canepari M, Filipis L. Blömer LA, et al. Curr Protoc. 2021 Mar;1(3):e64. doi: 10.1002/cpz1.64. Curr Protoc. 2021. PMID: 33657273
• Chemical Synthesis of Modified Oligonucleotides Containing 5'-Amino-5'-Deoxy-5'-Hydroxymethylthymidine Residues.

  Ohkubo A, Muto K, Watanabe R, Ogata D. Ohkubo A, et al. Curr Protoc. 2021 Mar;1(3):e70. doi: 10.1002/cpz1.70. Curr Protoc. 2021. PMID: 33657278
• Transformation of Borrelia burgdorferi.

  Seshu J, Moy BE, Ingle TM. Seshu J, et al. Curr Protoc. 2021 Feb;1(3):e61. doi: 10.1002/cpz1.61. Curr Protoc. 2021. PMID: 33661557
• Cardio Phenotypic Potential of Mesenchymal Stem Cells.

  Peterson KM, Franchi F, Olthoff M, Paulmurugan R, Rodriguez-Porcel M. Peterson KM, et al. Curr Protoc. 2021 Mar;1(3):e62. doi: 10.1002/cpz1.62. Curr Protoc. 2021. PMID: 33661576 Free PMC article.
• CRISPR-Cas9 Based Genome Editing in Wheat.

  Smedley MA, Hayta S, Clarke M, Harwood WA. Smedley MA, et al. Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65. Curr Protoc. 2021. PMID: 33687760
• VLP-factory™ and ADDomer^© : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display.

  Sari-Ak D, Bufton J, Gupta K, Garzoni F, Fitzgerald D, Schaffitzel C, Berger I. Sari-Ak D, et al. Curr Protoc. 2021 Mar;1(3):e55. doi: 10.1002/cpz1.55. Curr Protoc. 2021. PMID: 33729713 Free PMC article.
• Isolating and Analyzing Protein Containing Granules from Cells.

  Victor RA, Thompson VF, Schwartz JC. Victor RA, et al. Curr Protoc. 2021 Mar;1(3):e35. doi: 10.1002/cpz1.35. Curr Protoc. 2021. PMID: 33740275 Free PMC article.
• Imaging Spontaneous Neuronal Activity with Voltage-Sensitive Dyes.

  Raliski BK, Kirk MJ, Miller EW. Raliski BK, et al. Curr Protoc. 2021 Mar;1(3):e48. doi: 10.1002/cpz1.48. Curr Protoc. 2021. PMID: 33760396 Free PMC article.
• Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.

  Wang M, Shajahan A, Pepi LE, Azadi P, Zaia J. Wang M, et al. Curr Protoc. 2021 Mar;1(3):e84. doi: 10.1002/cpz1.84. Curr Protoc. 2021. PMID: 33761173 Free PMC article.