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. 2022 Jul 29;7(8):155.
doi: 10.3390/tropicalmed7080155.

Prevalence of Mutations in the Pfdhfr, Pfdhps, and Pfmdr1 Genes of Malarial Parasites Isolated from Symptomatic Patients in Dogondoutchi, Niger

Ibrahima Issa  1 , Mahaman Moustapha Lamine  2 , Veronique Hubert  3 , Amadou Ilagouma  4 , Eric Adehossi  4 , Aboubacar Mahamadou  1 , Neil F Lobo  5 , Demba Sarr  6 , Lisa M Shollenberger  7 , Houze Sandrine  3 , Ronan Jambou  1 , Ibrahim Maman Laminou  1
Affiliations

Prevalence of Mutations in the Pfdhfr, Pfdhps, and Pfmdr1 Genes of Malarial Parasites Isolated from Symptomatic Patients in Dogondoutchi, Niger

Ibrahima Issa et al. Trop Med Infect Dis. .

Abstract

The effectiveness of artemisinin-based combination therapies (ACTs) depends not only on that of artemisinin but also on that of partner molecules. This study aims to evaluate the prevalence of mutations in the Pfdhfr, Pfdhps, and Pfmdr1 genes from isolates collected during a clinical study. Plasmodium genomic DNA samples extracted from symptomatic malaria patients from Dogondoutchi, Niger, were sequenced by the Sanger method to determine mutations in the Pfdhfr (codons 51, 59, 108, and 164), Pfdhps (codons 436, 437, 540, 581, and 613), and Pfmdr1 (codons 86, 184, 1034, and 1246) genes. One hundred fifty-five (155) pre-treatment samples were sequenced for the Pfdhfr, Pfdhps, and Pfmdr1 genes. A high prevalence of mutations in the Pfdhfr gene was observed at the level of the N51I (84.97%), C59R (92.62%), and S108N (97.39%) codons. The key K540E mutation in the Pfdhps gene was not observed. Only one isolate was found to harbor a mutation at codon I431V. The most common mutation on the Pfmdr1 gene was Y184F in 71.43% of the mutations found, followed by N86Y in 10.20%. The triple-mutant haplotype N51I/C59R/S108N (IRN) was detected in 97% of the samples. Single-mutant (ICS and NCN) and double-mutant (IRS, NRN, and ICN) haplotypes were prevalent at 97% and 95%, respectively. Double-mutant haplotypes of the Pfdhps (581 and 613) and Pfmdr (86 and 184) were found in 3% and 25.45% of the isolates studied, respectively. The study focused on the molecular analysis of the sequencing of the Pfdhfr, Pfdhps, and Pfmdr1 genes. Although a high prevalence of mutations in the Pfdhfr gene have been observed, there is a lack of sulfadoxine pyrimethamine resistance. There is a high prevalence of mutation in the Pfmdr184 codon associated with resistance to amodiaquine. These data will be used by Niger's National Malaria Control Program to better monitor the resistance of Plasmodium to partner molecules in artemisinin-based combination therapies.

Keywords: Niger; P. falciparum; Pfdhfr; Pfdhps; Pfmdr1.

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Conflict of interest statement

The authors declare that there is no conflict of interest.

References

    1. WHO|Regional Office for Africa. Guidelines for the Treatment of Malaria. 3rd ed. [(accessed on 22 January 2022)]. Available online: https://www.afro.who.int/publications/guidelines-treatment-malaria-third....
    1. Etude de la Chimiorésistance de Plasmodium Falciparum Aux Antipaludiques de Première Intention et du Rôle de son Polymorphisme Dans la Symptomatology du Paludisme au Burkina. [(accessed on 22 January 2022)]. Available online: https://beep.ird.fr/collect/upb/index/assoc/INSSA-2017-SON-ETU/INSSA-201....
    1. Doudou M.H., Mahamadou A., Ouba I., Lazoumar R., Boubacar B., Arzika I., Zamanka H., Ibrahim M.L., Labbo R., Maiguizo S., et al. A refined estimate of the malaria burden in Niger. Malar. J. 2012;11:89. doi: 10.1186/1475-2875-11-89. - DOI - PMC - PubMed
    1. Ibrahima I., Laminou I.M., Adehossi E., Maman D., Boureima S., Harouna H.K., Hamidou H.H., Mahamadou A., Yacouba I., Hadiza J., et al. Safety and Efficacy of Artemether-Lumefantrine and Artesunate-Amodiaquine in Niger. Bull. Soc. Pathol. Exot. 2020;113:17–23. doi: 10.3166/bspe-2020-0120. - DOI - PubMed
    1. Noedl H., Socheat D., Satimai W. Artemisinin-Resistant Malaria in Asia. N. Engl. J. Med. 2009;361:540–541. doi: 10.1056/NEJMc0900231. - DOI - PubMed

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