Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Aug 18;9(8):439.
doi: 10.3390/vetsci9080439.

Importance of Antioxidant Supplementation during In Vitro Maturation of Mammalian Oocytes

Shimaa I Rakha  1   2 , Mohammed A Elmetwally  1   2 , Hossam El-Sheikh Ali  1   2 , Ahmed Balboula  1   2   3 , Abdelmonem Montaser Mahmoud  1   2 , Samy M Zaabel  1   2
Affiliations
Review

Importance of Antioxidant Supplementation during In Vitro Maturation of Mammalian Oocytes

Shimaa I Rakha et al. Vet Sci. .

Abstract

The in vitro embryo production (IVEP) technique is widely used in the field of reproductive biology. In vitro maturation (IVM) is the first and most critical step of IVEP, during which, the oocyte is matured in an artificial maturation medium under strict laboratory conditions. Despite all of the progress in the field of IVEP, the quality of in vitro matured oocytes remains inferior to that of those matured in vivo. The accumulation of substantial amounts of reactive oxygen species (ROS) within oocytes during IVM has been regarded as one of the main factors altering oocyte quality. One of the most promising approaches to overcome ROS accumulation within oocytes is the supplementation of oocyte IVM medium with antioxidants. In this article, we discuss recent advancements depicting the adverse effects of ROS on mammalian oocytes. We also discuss the potential use of antioxidants and their effect on both oocyte quality and IVM rate.

Keywords: IVEP; IVM; ROS; antioxidants; oocyte; oxidative stress.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic diagram summarizing the main factors causing oxidative stress of mammalian oocytes during their in vitro maturation. Consequences of oxidative stress on oocyte components are also shown. PO2, partial pressure of oxygen; ROS, reactive oxygen species. The diagram was created with BioRender.com [217].
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Baldassarre H. Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants. Animals. 2021;11:216. doi: 10.3390/ani11010216. - DOI - PMC - PubMed
    1. Wani N.A. In vitro embryo production (IVEP) in camelids, Present status and future perspectives. Reprod. Biol. 2021;21:100471. doi: 10.1016/j.repbio.2020.100471. - DOI - PubMed
    1. Baldassarre H. Practical aspects for implementing in vitro embryo production and cloning programs in sheep and goats. Anim. Reprod. 2018;9:188–194.
    1. Hatırnaz Ş., Ata B., Hatırnaz E.S., Dahan M.H., Tannus S., Tan J., Tan S.L. Oocyte in vitro maturation, A sytematic review. Turk. J. Obstet. Gynecol. 2018;15:112–125. doi: 10.4274/tjod.23911. - DOI - PMC - PubMed
    1. Mikkelsen A.L., Smith S., Lindenberg S. Possible factors affecting the development of oocytes in in-vitro maturation. Hum. Reprod. 2000;15((Suppl. 5)):11–17. doi: 10.1093/humrep/15.suppl_5.11. - DOI - PubMed

LinkOut - more resources