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. 2022 Aug 25;17(8):e0272901.
doi: 10.1371/journal.pone.0272901. eCollection 2022.

Expression of Concern: Deletion of the Mitochondrial Flavoprotein Apoptosis Inducing Factor (AIF) Induces β-Cell Apoptosis and Impairs β-Cell Mass

PLOS ONE Editors

Expression of Concern: Deletion of the Mitochondrial Flavoprotein Apoptosis Inducing Factor (AIF) Induces β-Cell Apoptosis and Impairs β-Cell Mass

PLOS ONE Editors. PLoS One. .
No abstract available

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Figures

Fig 3
Fig 3. AIF depletion leads to increased β-cell apoptosis in human islets without affecting insulin secretion.
(A) Isolated human pancreatic islets were exposed to siRNA to AIF (siAIF) or scrambled control siRNA (siScr) for 3 days. The knockdown efficiency was determined by Western blot analysis. Actin was used as a loading control on the same membrane after stripping. This Western blot is representative of three independent experiments from three different organ donors. The density of expression levels were quantified after scanning and normalised to actin levels. (B–D) 3 days after transfection, islets were exposed to 50 μM H2O2 for 2 h. (B) Islet sections were prepared for analysis of β-cell apoptosis by the TUNEL assay. Islets were double-stained for insulin in green and counterstained for DAPI in blue. Results are means ± SE of the percentage of TUNEL-positive β-cells. The mean number of β-cells counted was 3400 for each treatment condition. (C,D) GSIS: after the H2O2 treatment, islets were washed and basal and stimulated insulin secretion analyzed during successive 1-h incubations at 2.8 mM (basal) and 16.7 mM (stimulated) glucose. Data are normalized to insulin content. (D) Stimulatory index denotes the ratio between stimulated and basal values of insulin secretion. (B–D) All assays were performed in triplicate or quadruplicate in three independent experiments from 3 different organ donors, respectively. *p<0.05 to siScr control, **p<0.05 in H2O2 treated vs. untreated control.
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Expression of concern for

References

    1. Schulthess FT, Katz S, Ardestani A, Kawahira H, Georgia S, Bosco D, et al. (2009) Deletion of the Mitochondrial Flavoprotein Apoptosis Inducing Factor (AIF) Induces β-Cell Apoptosis and Impairs β-Cell Mass. PLoS ONE 4(2): e4394. 10.1371/journal.pone.0004394 - DOI - PMC - PubMed
    1. Maedler Kathrin, Schumann Desiree M., Sauter Nadine, Ellingsgaard Helga, Bosco Domenico, Baertschiger Reto, Iwakura Yoichiro, José Oberholzer, Claes B. Wollheim Benoit R. Gauthier Marc Y. Donath. (2006); Low Concentration of Interleukin-1β Induces FLICE-Inhibitory Protein–Mediated β-Cell Proliferation in Human Pancreatic Islets. Diabetes 55 (10): 2713–2722. 10.2337/db05-1430 - DOI - PubMed
    1. Statement of Retraction. (2020) Kathrin Maedler, Desiree M. Schumann, Nadine Sauter, Helga Ellingsgaard, Domenico Bosco, Reto Baertschiger, Yoichiro Iwakura, José Oberholzer, Claes B. Wollheim, Benoit R. Gauthier, and Marc Y. Donath. Low Concentration of Interleukin-1β Induces FLICE-Inhibitory Protein–Mediated β-Cell Proliferation in Human Pancreatic Islets. Diabetes 55:2713–2722. doi: 10.2337/db05-1430 . Diabetes 69 (3): 493. 10.2337/db20-rt03a - DOI - PubMed
    1. The PLOS ONE Staff (2015) Correction: Deletion of the Mitochondrial Flavoprotein Apoptosis Inducing Factor (AIF) Induces β-Cell Apoptosis and Impairs β-Cell Mass. PLoS ONE 10(5): e0117766. doi: 10.1371/journal.pone.0117766 - DOI - PMC - PubMed

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