doi: 10.1038/275617a0.
Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase
- PMID: 360074
- DOI: 10.1038/275617a0
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Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase
Nature.
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Abstract
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.
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