[Screening, domestication and identification of intestinal uric acid degrading bacteria in low uric acid population]

Abstract

As the largest ecosystem of human body, intestinal microorganisms participate in the synthesis and metabolism of uric acid. Developing and utilizing intestinal bacteria to degrade uric acid might provide new ideas for the treatment of hyperuricemia. The fecal samples of people with low uric acid were inoculated into uric acid selective medium with the concentration of 1.5 mmol/L for preliminary screening, and the initially screened strains that may have degradation ability were domesticated by concentration gradient method, and the strains with high uric acid degradation rate were identified by 16S rRNA sequencing method. A strain of high-efficiency uric acid degrading bacteria was screened and domesticated from the feces of people with low uric acid. The degradation rate of uric acid could reach 50.2%. It was identified as Escherichia coli. The isolation and domestication of high efficient uric acid degrading strains can not only provide scientific basis for the study of the mechanism of intestinal microbial degradation of uric acid, but also reserve biological strains for the treatment of hyperuricemia and gout in the future.

Keywords: Domestication; Low uric acid population; Screen; Uric acid degradation rate; Uric acid degrading bacteria.

图 1

Standard curve of different concentrations of uric acid standard 不同浓度尿酸标准品标准曲线

图 2

Colonial morphology of strain S3A S3A菌落形态

图 3

Morphology of strain S3A under the microscope after Gram staining (10 × 100) S3A革兰染色显微镜下形态（10 × 100）

图 4

16S rRNA PCR amplification results 16S rRNA PCR扩增结果

图 5

System evolution tree of S3A S3A株系统进化树