Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2542:271-285.
doi: 10.1007/978-1-0716-2549-1_20.

Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model

Jesús A Romo  1 , Carol A Kumamoto  2
Affiliations

Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model

Jesús A Romo et al. Methods Mol Biol. 2022.

Abstract

The role of fungal colonizers of the gastrointestinal tract during disease states is not well understood. Antibiotic treatment renders patients highly susceptible to infection by the bacterial pathogen C. difficile while also leading to blooms in fungal commensals, setting the stage for trans-kingdom interactions. Here, we describe a murine model of Candida gastrointestinal colonization coupled to a C. difficile infection (CDI) model, the measurement of CFU of both organisms, and collection of cecum and colon contents for the purpose of quantifying C. difficile toxin production. Additionally, we describe how to induce and purify C. difficile spores.

Keywords: Candida albicans; Candida glabrata; Clostridioides difficile; Gastrointestinal colonization; Murine model.

PubMed Disclaimer

References

    1. Huffnagle GB, Noverr MC (2013) The emerging world of the fungal microbiome. Trends Microbiol 21(7):334–341 - PubMed - PMC
    1. Goodrich JK et al (2014) Conducting a microbiome study. Cell 158(2):250–262 - PubMed - PMC
    1. Huseyin CE et al (2017) The fungal frontier: a comparative analysis of methods used in the study of the Human Gut Mycobiome. Front Microbiol 8:1432 - PubMed - PMC
    1. Hallen-Adams HE et al (2015) Fungi inhabiting the healthy human gastrointestinal tract: a diverse and dynamic community. Fungal Ecol 15:9–17
    1. Hallen-Adams HE, Suhr MJ (2017) Fungi in the healthy human gastrointestinal tract. Virulence 8(3):352–358 - PubMed

Publication types

LinkOut - more resources