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. 2022 Aug 16;11(8):1586.
doi: 10.3390/antiox11081586.

Pre-Treatment Physical Activity Could Positively Influence Pregnancy Rates in IVF despite the Induced Oxidative Stress: A Cohort Study on Salivary 8-Hydroxy-2'-deoxyguanosine

Viktória Prémusz  1   2   3 , Dominika Lendvai-Emmert  4   5 , Alexandra Makai  2 , Krisztina Amrein  5   6 , Shalini Chauhan  4 , József Bódis  1   3 , Kálmán András Kovács  7 , Ákos Várnagy  1   3   7
Affiliations

Pre-Treatment Physical Activity Could Positively Influence Pregnancy Rates in IVF despite the Induced Oxidative Stress: A Cohort Study on Salivary 8-Hydroxy-2'-deoxyguanosine

Viktória Prémusz et al. Antioxidants (Basel). .

Abstract

(1) Background: This study was designed to define whether pretreatment habitual physical activity (PA)-induced oxidative stress (OS) influences outcome measures by using 8-hydroxy-2′-deoxyguanosine (8-OHdG) in saliva samples of patients undergoing in vitro fertilization (IVF). (2) Method: In this cohort study, samples were obtained from 26 patients (age: 34.6 ± 5.5 years, BMI: 25.3 ± 5.1, infertility: 51.0 ± 28.7 months) before the treatment and a follow-up of outcome measures of IVF/ICSI. The 8-OHdG was evaluated by Abcam’s ELISA (ab201734), PA patterns by GPAQ-H and ActiGraph GT3X; (3) Results: The number of matured oocytes was positively influenced by the GPAQ-H recreation MET (R2 = 0.367, F = 10.994, p = 0.004; β = 0.005, p = 0.004, B Constant = 4.604) and a positive significant relationship (R2 = 0.757, F = 17.692, p < 0.001, B Constant = 1.342) was found with GPAQ-H recreational PA MET (β = 0.004, p < 0.001), and Grade 1 embryos and higher very vigorous activity (GT3X) were accompanied (R2 = 0.958, F = 408.479, p < 0.001) by higher ß-hCG levels (β = 63.703, p ≤ 0.001). Unanticipated positive correlation between 8-OHdG and ß-hCG level (R = 0.467, p = 0.028) was noticed, and there were significant differences in 8-OHdG in biochemical pregnancies (pregnant: 54.82 ± 35.56 ng/mL, non-pregnant: 30.06 ± 10.40 ng/mL, p = 0.022) as well. (4) Conclusions: Pretreatment PA could positively influence reproductive performance in IVF/ICSI despite the induced OS. However, a more sensitive biomarker and the recommended amount of activity should be further investigated.

Keywords: 8-hydroxy-2′-deoxyguanosine; DNA; fertilization in vitro; oxidative stress; physical activity.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Flow diagram of patients’ enrolment.
Figure 2
Figure 2
Differences between the 8-hydroxy-2′-deoxyguanosine (8-OHdG) ng/mL levels in patients undergoing IVF using saliva samples. E: evening, M: morning, number of matured oocytes over 6. * p ˂ 0.05.
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References

    1. WHO . Global Action Plan on Physical Activity 2018–2030: More Active People for a Healthier World. World Health Organization; Geneva, Switzerland: 2018.
    1. Ács P., Stocker M., Paár D., Oláh A., Kovács A. Economic and public health benefits: The result of increased regular physical activity. Eur. J. Integr. Med. 2016;8:8–12. doi: 10.1016/j.eujim.2016.11.003. - DOI
    1. Pizzino G., Irrera N., Cucinotta M., Pallio G., Mannino F., Arcoraci V., Squadrito F., Altavilla D., Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxid. Med. Cell. Longev. 2017;2017:8416763. doi: 10.1155/2017/8416763. - DOI - PMC - PubMed
    1. Palomba S., Falbo A., Valli B., Morini D., Villani M.T., Nicoli A., La Sala G.B. Physical activity before IVF and ICSI cycles in infertile obese women: An observational cohort study. Reprod. Biomed. Online. 2014;29:72–79. doi: 10.1016/j.rbmo.2014.03.006. - DOI - PubMed
    1. Rao M., Zeng Z., Tang L. Maternal physical activity before IVF/ICSI cycles improves clinical pregnancy rate and live birth rate: A systematic review and meta-analysis. Reprod. Biol. Endocrinol. 2018;16:11. doi: 10.1186/s12958-018-0328-z. - DOI - PMC - PubMed

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