Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Aug 5;13(8):1394.
doi: 10.3390/genes13081394.

Single Nucleotide Polymorphisms in Amlodipine-Associated Genes and Their Correlation with Blood Pressure Control among South African Adults with Hypertension

Charity Masilela  1 , Oladele Vincent Adeniyi  2 , Mongi Benjeddou  3
Affiliations

Single Nucleotide Polymorphisms in Amlodipine-Associated Genes and Their Correlation with Blood Pressure Control among South African Adults with Hypertension

Charity Masilela et al. Genes (Basel). .

Abstract

Objective: This study describes the single nucleotide polymorphisms (SNPs) in amlodipine-associated genes and assesses their correlation with blood pressure control among South African adults with hypertension. Methods: In total, 304 hypertensive patients on amlodipine treatment belonging to the indigenous Swati, Xhosa and Zulu population groups of South Africa were recruited between June 2017 and June 2019. Participants were categorized into: controlled (blood pressure < 140/90 mmHg) and uncontrolled (blood pressure ≥ 140/90 mmHg) hypertension. Thirteen SNPs in amlodipine pharmacogenes with a high PharmGKB evidence base were selected and genotyped using MassArray (Agena BioscienceTM). Logistic regression was fitted to identify significant associations between the SNPs and blood pressure control with amlodipine. Results: The majority of the participants were females (76.6%), older than 45 years (89.1%) and had uncontrolled hypertension (52.3%). Of the 13 SNPs genotyped, five SNPs, rs1042713 (minor allele frequency = 45.9%), rs10494366 (minor allele frequency = 35.3%), rs2239050 (minor allele frequency = 28.7%), rs2246709 (minor allele frequency = 51.6%) and rs4291 (minor allele frequency = 34.4%), were detected among the Xhosa participants, while none were detected among the Swati and Zulu tribal groups. Variants rs1042713 and rs10494366 demonstrated an expression frequency of 97.5% and 79.5%, respectively. Variant TA genotype of rs4291 was significantly associated with uncontrolled hypertension. No association was established between blood pressure response to amlodipine and the remaining four SNPs. Conclusions: This study reports the discovery of five SNPs in amlodipine genes (rs2239050, rs2246709, rs4291, rs1042713 and rs10494366) among the indigenous Xhosa-speaking tribe of South Africa. In addition, the TA genotype of rs4291 was associated with blood pressure control in this cohort. These findings might open doors for more pharmacogenomic studies, which could inform innovations to personalised anti-hypertensive treatment in the ethnically diverse population of South Africa.

Keywords: South Africa; amlodipine; single nucleotide polymorphisms; uncontrolled hypertension.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Jongen V.W., Lalla-Edward S.T., Vos A.G., Godijk N.G., Tempelman H., Grobbee D.E., Devillé W., Klipstein-Grobusch K. Hypertension in a rural community in South Africa: What they know, what they think they know and what they recommend. BMC Public Health. 2019;19:341. doi: 10.1186/s12889-019-6642-3. - DOI - PMC - PubMed
    1. National Department of Health (NDoH) Statistics South Africa (Stats SA) South African Medical Research Council (SAMRC) ICF . South Africa Demographic and Health Survey 2016. NDoH, Stats SA, SAMRC, and ICF; Pretoria, South Africa: 2019.
    1. Monakali S., Ter Goon D., Seekoe E., Owolabi E.O. Prevalence, awareness, control and determinants of hypertension among primary health care professional nurses in Eastern Cape, South Africa. Afr. J. Prim. Health Care Fam. Med. 2018;10:e1–e5. doi: 10.4102/phcfm.v10i1.1758. - DOI - PMC - PubMed
    1. Mkhonto S.S., Labadarios D., Mabaso M.L. Association of body weight and physical activity with blood pressure in a rural population in the Dikgale village of Limpopo Province in South Africa. BMC Res. Notes. 2012;5:118. doi: 10.1186/1756-0500-5-118. - DOI - PMC - PubMed
    1. Prakaschandra D.R., Esterhuizen T.M., Motala A.A., Gathiram P., Naidoo D.P. High prevalence of cardiovascular risk factors in Durban South African Indians: The Phoenix Lifestyle Project. S. Afr. Med. J. 2016;106:284–289. doi: 10.7196/SAMJ.2016.v106i3.9837. - DOI - PubMed

Publication types