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Review
. 2022 Aug 5;10(8):1573.
doi: 10.3390/microorganisms10081573.

Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections

Giulia Gatti  1 , Francesca Taddei  1 , Martina Brandolini  1 , Andrea Mancini  1 , Agnese Denicolò  1 , Francesco Congestrì  1 , Martina Manera  1 , Valentina Arfilli  1 , Arianna Battisti  1 , Silvia Zannoli  1 , Maria Michela Marino  1 , Anna Marzucco  1 , Manuela Morotti  1 , Laura Grumiro  1 , Agata Scalcione  1 , Giorgio Dirani  1 , Monica Cricca  1   2 , Vittorio Sambri  1   2
Affiliations
Review

Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections

Giulia Gatti et al. Microorganisms. .

Abstract

The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.

Keywords: NGS; PCR; PJI; molecular diagnosis; prosthetic joint infection.

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Conflict of interest statement

The authors declare no conflict of interest.

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