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. 2022 Aug 21;11(16):2172.
doi: 10.3390/plants11162172.

Construction of A GBS-Based High-Density Genetic Map and Flower Color-Related Loci Mapping in Grasspea (Lathyrus sativus L.)

Xiaopeng Hao  1   2   3   4 , Tao Yang  5 , Yan Wang  2   3   4 , Rong Liu  5 , Xue Dong  2   3   4 , Jiandong Zhao  2   3   4 , Jucai Han  1 , Xuxiao Zong  5 , Jianwu Chang  2   3   4 , Huiping Liu  1
Affiliations

Construction of A GBS-Based High-Density Genetic Map and Flower Color-Related Loci Mapping in Grasspea (Lathyrus sativus L.)

Xiaopeng Hao et al. Plants (Basel). .

Abstract

Grasspea (Lathyrus sativus L.), a legume crop with excellent resistance to a broad array of environmental stressors, has, to this point, been poorly genetically characterized. High-density genetic linkage maps are critical for draft genome assembly, quantitative trait loci (QTLs) analysis, and gene mining. The lack of a high-density genetic linkage map has limited both genomic studies and selective breeding in grasspea. Here, we developed a high-density genetic linkage map of grasspea using genotyping-by-sequencing (GBS) to sequence 154 grasspea plants, comprising 2 parents and 152 F2 progeny. In all, 307.74 Gb of data was produced, including 2,108,910,938 paired-end reads, as well as 3536 SNPs mapped to seven linkage groups (LG1-LG7). With an average length of 996.52 cM per LG, the overall genetic distance was 6975.68 cM. Both the χ2 test and QTL analysis, based on the Kruskal-Wallis (KW) test and interval mapping (IM) analysis, revealed the monogenic inheritance of flower color in grasspea, with the responsible QTL located between 308.437 cM and 311.346 cM in LG4. The results can aid grasspea genome assembly and accelerate the selective breeding of new grasspea germplasm resources.

Keywords: Lathyrus sativus L. (grasspea); QTLs; SNP; genetic linkage map; genotyping-by-sequencing (GBS).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Frequency of polymorphism segregation types between K714 and IF1347. Different colors (from left to right) represent the 7 segregation types.
Figure 2
Figure 2
Kruskal–Wallis (KW) analysis (p < 0.01). The grey arrow and blue point indicate the most significant value. Chromosomes (left to right, from 1 to 7) are represented with different colors along the x-axis.
Figure 3
Figure 3
Interval mapping (IM) test (LOD > 3.0). The grey arrow and green point indicate the most significant value. Chromosomes (left to right) are represented from 1 to 7 along the x-axis.
See this image and copyright information in PMC

References

    1. Narayan R.K.J. Discontinuous DNA variation in the evolution of plant species—The genus Lathyrus. Evol. 1982;36:877–891. doi: 10.1111/j.1558-5646.1982.tb05460.x. - DOI - PMC - PubMed
    1. Roy B. On the somatic chromosomes in Lathyrus. Cytologia. 1936;7:424–430. doi: 10.1508/cytologia.7.424. - DOI
    1. Talukdar D. Cytogenetic characterization of induced autotetraploids in grass pea (Lathyrus sativus L.) Caryologia. 2010;63:62–72.
    1. Verma S.C., Ohri D. Chromosome and nucclear phenotype in the legume Lathyrus sativus L. Cytologia. 1979;44:77–90. doi: 10.1508/cytologia.44.77. - DOI
    1. Kislev M.E. Origins of the cultivation of Lathyrus sativus and L. cicera (fabaceae) Econ. Bot. 1989;43:262–270. doi: 10.1007/BF02859868. - DOI

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