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. 1987 Jul 10;15(13):5353-72.
doi: 10.1093/nar/15.13.5353.

Covalent attachment of oligonucleotides to solid supports

S S Ghosh, G F Musso
Free PMC article

Covalent attachment of oligonucleotides to solid supports

S S Ghosh et al. Nucleic Acids Res. .
Free PMC article

Abstract

Coupling efficiencies for the covalent attachment of oligonucleotides (17-29 bases in length) to solid supports derivatized with alkyl-amino and -carboxylic functionalities have been determined. Attachment efficiencies of 60-80% were obtained for coated long-chain alkylamino controlled pore glass (CPG) supports. Similar efficiencies of immobilization were observed for carboxyl-bearing supports, which additionally exhibited lower levels of non-covalent binding. The extent of terminally linked oligonucleotide was determined to be 50-55% of the overall attachment in the carbodiimide-mediated coupling reaction of a 5'-aminohexyl phosphoramidate derivative of a 29-mer to Sephacryl carboxyl support. While lower overall efficiencies of attachment were obtained in the reaction with Sephacryl N-hydroxysuccinimide-activated carboxyl support, greater than 80% of this coupling results in end-attached oligonucleotides.

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References

    1. Anal Biochem. 1970 Apr;34(2):595-8 - PubMed
    1. Nucleic Acids Res. 1987 Apr 10;15(7):3131-9 - PubMed
    1. Anal Chim Acta. 1972 Jan;58(1):248-9 - PubMed
    1. J Biol Chem. 1974 Aug 25;249(16):5213-21 - PubMed
    1. Cell. 1975 Jul;5(3):301-10 - PubMed