Apurinic endonucleases from Saccharomyces cerevisiae
- PMID: 360170
- PMCID: PMC342254
- DOI: 10.1093/nar/5.9.3347
Apurinic endonucleases from Saccharomyces cerevisiae
Abstract
Three endonuclease activities have been partially purified from Saccharomyces cerevisiae on the basis of their activity against x-irradiated closed-circular supercoiled bacteriophage PM2 DNA. These endonucleases also nick apurinic DNA and two out of the three activities incise DNA UV-irradiated with high doses. The endonuclease activities have also been distinguished on the basis of their magnesium requirement and sensitivity to EDTA.
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