. 2023 Jun;201(6):2942-2954.

doi: 10.1007/s12011-022-03399-w. Epub 2022 Aug 26.

Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in Liver and Kidney Tissues: Potential Protective Role of Thymoquinone

Basma Salama¹, Khalid J Alzahrani², Khalid S Alghamdi³, Osama Al-Amer^{4

5}, Khalid E Hassan⁶, Mohamed A Elhefny^{7

8}, Alaa Jameel A Albarakati⁹, Fahad Alharthi¹⁰, Hussam A Althagafi¹¹, Hassan Al Sberi^{12

13}, Hatem K Amin¹⁴, Maha S Lokman¹⁵, Khalaf F Alsharif², Ashraf Albrakati¹⁶, Ahmed E Abdel Moneim¹⁷, Rami B Kassab^{18

19}, Ayah S Fathalla¹⁷

Affiliations

¹ Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
² Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
³ Regional Laboratory, General Administration of Laboratories and Blood Banks, Ministry of Health, Taif, Saudi Arabia.
⁴ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁵ Genome and Biotechnology Unit, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁶ Pathology Department, College of Medicine, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
⁷ Department of Cancer and Molecular Biology, National Cancer Institute, Cairo University, Cairo, Egypt.
⁸ Department of Medial Genetics, Faculty of Medicine, Umm Al-Qura University, Alqunfudah, Saudi Arabia.
⁹ Surgery Department, College of Medicine, Al-Qunfudah Branch, Umm Al-Qura University, Makkah, Saudi Arabia.
¹⁰ Department of Biology, College of Science, Taif University, Taif, Saudi Arabia.
¹¹ Department of Biology, Faculty of Science and Arts, Al-Baha University, Almakhwah, Al-Baha, Saudi Arabia.
¹² Basic Medical Science, Histopathology Department, National Organization for Drug Control and Research, Giza, Egypt.
¹³ Department of Biology, Faculty of Science, Taif University, Taif, Saudi Arabia.
¹⁴ Biochemistry Department, Faculty of Pharmacy, Galala University, El-Galala City, Egypt.
¹⁵ Biology Department, College of Science and Humanities, Prince Sattam Bin Abdul Aziz University, Alkharj, Saudi Arabia.
¹⁶ Department of Human Anatomy, College of Medicine, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
¹⁷ Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, 11795, Egypt.
¹⁸ Department of Biology, Faculty of Science and Arts, Al-Baha University, Almakhwah, Al-Baha, Saudi Arabia. rami.kassap@yahoo.com.
¹⁹ Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, 11795, Egypt. rami.kassap@yahoo.com.

PMID: 36018545
DOI: 10.1007/s12011-022-03399-w

Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in Liver and Kidney Tissues: Potential Protective Role of Thymoquinone

Basma Salama et al. Biol Trace Elem Res. 2023 Jun.

. 2023 Jun;201(6):2942-2954.

doi: 10.1007/s12011-022-03399-w. Epub 2022 Aug 26.

Authors

Affiliations

¹ Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
² Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
³ Regional Laboratory, General Administration of Laboratories and Blood Banks, Ministry of Health, Taif, Saudi Arabia.
⁴ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁵ Genome and Biotechnology Unit, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁶ Pathology Department, College of Medicine, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
⁷ Department of Cancer and Molecular Biology, National Cancer Institute, Cairo University, Cairo, Egypt.
⁸ Department of Medial Genetics, Faculty of Medicine, Umm Al-Qura University, Alqunfudah, Saudi Arabia.
⁹ Surgery Department, College of Medicine, Al-Qunfudah Branch, Umm Al-Qura University, Makkah, Saudi Arabia.
¹⁰ Department of Biology, College of Science, Taif University, Taif, Saudi Arabia.
¹¹ Department of Biology, Faculty of Science and Arts, Al-Baha University, Almakhwah, Al-Baha, Saudi Arabia.
¹² Basic Medical Science, Histopathology Department, National Organization for Drug Control and Research, Giza, Egypt.
¹³ Department of Biology, Faculty of Science, Taif University, Taif, Saudi Arabia.
¹⁴ Biochemistry Department, Faculty of Pharmacy, Galala University, El-Galala City, Egypt.
¹⁵ Biology Department, College of Science and Humanities, Prince Sattam Bin Abdul Aziz University, Alkharj, Saudi Arabia.
¹⁶ Department of Human Anatomy, College of Medicine, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.
¹⁷ Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, 11795, Egypt.
¹⁸ Department of Biology, Faculty of Science and Arts, Al-Baha University, Almakhwah, Al-Baha, Saudi Arabia. rami.kassap@yahoo.com.
¹⁹ Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, 11795, Egypt. rami.kassap@yahoo.com.

PMID: 36018545
DOI: 10.1007/s12011-022-03399-w

Abstract

Silver nanoparticles (AgNPs) are the most common nanomaterials in consumer products. Therefore, it has been crucial to control AgNPs toxicological effects to improve their safety and increase the outcome of their applications. This work investigated the possible protective effect of thymoquinone (TQ) against AgNPs-induced hepatic and renal cytotoxicity in rats. Serum markers of liver and kidney functions as well as liver and kidney oxidative stress status, pro-inflammatory cytokines, apoptosis markers, and histopathology were assessed. TQ reversed AgNPs-induced elevation in serum liver and kidney function markers, including aspartate transaminase, alanine transaminase, urea, and creatinine. Moreover, TQ co-administration with AgNPs alleviates hepatic and renal oxidative insults by decreasing MDA and NO levels with a significant increase in the activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione recycling enzymes peroxidase and reductase) compared to AgNPs-treated rats. Besides, TQ upregulated hepatic and renal Nrf2 gene expression in AgNPs-intoxicated rats. Furthermore, TQ co-administration decreased the hepatic and renal pro-inflammatory mediators represented by IL-1β, TNF-α, TGF-β, and NF-κB levels. Besides, TQ co-administration decreased apoptotic protein (Bax) levels and increased the anti-apoptotic protein (Bcl-2) levels. These findings were confirmed by the histopathological examination of hepatic and renal tissues. Our data affirmed the protective effect of TQ against AgNPs cytotoxicity and proposed a possible mechanism of TQ antioxidant, anti-inflammatory, and anti-apoptotic effects. Consequently, we could conclude that using TQ might control AgNPs toxicological effects, improve their safety, and increase the outcome of their applications.

Keywords: Apoptosis; Hepatorenal Toxicity; Inflammation; Oxidative Damage; Silver Nanoparticles; ThyMoquinone.

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Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in Liver and Kidney Tissues: Potential Protective Role of Thymoquinone

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Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in Liver and Kidney Tissues: Potential Protective Role of Thymoquinone

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