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. 1978 Sep;75(9):4296-300.
doi: 10.1073/pnas.75.9.4296.

Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils

T Wallimann, G Pelloni, D C Turner, H M Eppenberger

Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils

T Wallimann et al. Proc Natl Acad Sci U S A. 1978 Sep.

Abstract

Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle myofibrils. Incubation of myofibrils with monovalent Fab' fragments of these antibodies solubilizes most of the myofibril-bound creatine kinase, concomitantly removing most of the electron-dense material from the M lines. This strongly indicates that MM-CK is an integral part of the M-line structure and is consistent with the suggestion that MM-CK molecules form the M bridges that are responsible for the principle M-line substriations.

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