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. 2022 Jun 7:42:100.
doi: 10.11604/pamj.2022.42.100.30531. eCollection 2022.

Detection and quantitation of hepatitis B surface antigen (HBsAg) on dried blood spots: a solution for easy access for hepatitis B diagnosis and elimination in remote areas

Anna Julienne Selbé Ndiaye  1   2 , Halimatou Diop-Ndiaye  1 , Gora Lo  1   2 , Aminata Dia  2 , Mba El Hadji Bambo Diakhaby  1 , Aissatou Sow  1   2 , Assane Dieng  1 , Souleymane Mboup  2 , Cheikh Saad Bouh Boye  1 , Coumba Touré Kane  2
Affiliations

Detection and quantitation of hepatitis B surface antigen (HBsAg) on dried blood spots: a solution for easy access for hepatitis B diagnosis and elimination in remote areas

Anna Julienne Selbé Ndiaye et al. Pan Afr Med J. .

Abstract

Hepatitis B virus (HBV) is generally endemic in resource-limited countries, which are characterized by a deficit of technical facilities that could delay diagnosis and treatment. To facilitate the accessibility to diagnostic and connection to treatment, evaluation, and promotion of alternatives and/or simplified strategies and inexpensive tools such as dried blood specimens need to be investigated and implemented. This study aimed to evaluate dried blood spots (DBS) for the detection and quantification of HBsAg. This study included 100 DBS from subjects tested positive for HBsAg, and 50 DBSs from subjects tested negative for HBsAg by the automate Architect i1000sr (Abbott Diagnostics, Ireland). Hepatitis B surface antigen detection was performed with determine HBsAg Alere® tests (Alere International Limited, Ireland) and Architect® HBsAg Qualitative II Assays (Abbott, Diagnostics, Ireland) after 15 and 30 days (D15, D30). For HBsAg-positive subjects, the quantification of HBsAg was performed at day zero (D0) from plasma and at D15 and D30 from the DBSs. At D15, the sensitivity and specificity were 96% and 100% for the Determine® tests and 100% and 100% for the Architect® tests, respectively. At D30, the sensitivity and specificity were 96% and 100% for the Determine® tests and 100% and 100% for the Architect® tests, respectively. For HBsAg quantification, the agreement rates were 96%, 96% and 100% between D0-D15, D0-D30 and D15-D30, respectively. This work showed that DBSs can be very useful for HBsAg detection and quantification and therefore in the management of HBV infection in resource-limited settings.

Keywords: HBsAg quantification; Hepatitis B virus; dried blood spots.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
regression line and Bland-Altman plots for quantitated HBsAg from plasma at day 0 versus dried blood spots at day 15
Figure 2
Figure 2
regression line and Bland-Altman plot for quantitated HBsAg from plasma at day 0 versus dried blood spots at day 30
Figure 3
Figure 3
regression line and Bland-Altman plots for quantitated HBsAg from dried blood spots at day 15 versus day 30
See this image and copyright information in PMC

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