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. 2022 Aug 12:13:954867.
doi: 10.3389/fphar.2022.954867. eCollection 2022.

Taohong siwu decoction attenuates AIM2 and NLRC4 inflammasomes by ameliorates deoxyribonucleic acid damage after ischemic stroke

Ni Wang  1   2   3   4   5 , Furui Chu  1   2   3   4   5 , Lijuan Zhang  1   2 , Changyi Fei  1   2 , Chao Yu  1   2 , Sujun Xue  1   2 , Yongzhong Wang  1 , Ling Fang  6 , Daiyin Peng  2   3   4 , Xianchun Duan  1   2   3   4   5 , Weidong Chen  2   3   4
Affiliations

Taohong siwu decoction attenuates AIM2 and NLRC4 inflammasomes by ameliorates deoxyribonucleic acid damage after ischemic stroke

Ni Wang et al. Front Pharmacol. .

Abstract

Taohong siwu decoction (THSWD) has been shown to have a therapeutic effect on ischemic strokes (IS). However, it is not clear to us whether THSWD reduces deoxyribonucleic acid (DNA) damage after stroke and reduces the inflammatory response caused by the damage. Therefore, we constructed an IS model (I/R) in rats and performed oxygen-glucose deprivation/reoxygenation (OGD/R) on BV2 cells. Then ELISA, immunofluorescence staining, immunohistochemistry staining, and RT-qPCR were performed to detect the expressions of absent in melanoma 2 (AIM2), NLRC4, and Caspase-1 inflammasomes and other inflammatory factors. Experimental stroke causes DNA damage, and we found that the aforementioned inflammasomes as well as inflammatory factors were significantly inhibited after treatment with THSWD by comparing the model group with the model administration group. In addition, we examined the expression of AIM2, NLRC4, and Caspase-1 in BV2 cells of OGD/R and found that the expression of the aforementioned inflammasomes was significantly decreased in OGD/R by administration of THSWD-containing serum. Our data suggest that THSWD can reduced DNA damage after stroke as well as the inflammatory response caused by the damage.

Keywords: AIM2; NLRC4; Taohong Siwu Decoction; inflammasomes; ischemic stroke.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Neurofunctional score analysis of taohong siwu decoction (THSWD). Notes: *p < 0.05 vs. Sham group; # p < 0.05 vs. I/R group.
FIGURE 2
FIGURE 2
Inhibition rate and different concentrations of THSWD-containing serum. Notes: (A) inhibition rate (B) cell viability.
FIGURE 3
FIGURE 3
Expression of absent in melanoma 2 (AIM2), NLRC4, and caspase-1 inflammasomes in animal models. Notes: # p < 0.01 vs. Sham group; ## p < 0.01 vs. I/R group.
FIGURE 4
FIGURE 4
Expression of AIM2, NLRC4 and caspase-1 inflammasomes in cellular models. Notes: # p < 0.01 vs. Sham group; ## p < 0.01 vs. I/R group.
FIGURE 5
FIGURE 5
ELISA analysis of THSWD. Notes: # p < 0.01 vs. Sham group; ## p < 0.01 vs. I/R group.
FIGURE 6
FIGURE 6
mRNA expression of IL-1β and IL-18. Notes: *p < 0.05, # p < 0.01 vs. Sham group; **p < 0.05 vs. I/R group.
FIGURE 7
FIGURE 7
Expression of γ-H2AX in each group. Notes: # p < 0.01 vs. Sham group; **p < 0.05 vs. I/R group.
FIGURE 8
FIGURE 8
DNA) testing. Notes: (A) animal models (B) cellular models. A *p < 0.05, # p < 0.01 vs. Sham group; **p < 0.05 vs. I/R group. B *p < 0.05, # p < 0.01 vs. CN group; **p < 0.05, ## p < 0.01 vs. oxygen-glucose deprivation/reoxygenation (OGD/R) group.
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