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. 2022 Aug 21;19(9):1482-1501.
doi: 10.7150/ijms.73404. eCollection 2022.

Comprehensive Analysis of Transcriptional Expression of hsa-mir-21 Predicted Target Genes and Immune Characteristics in Kidney Renal Clear Cell Carcinoma

Da-Ming Xu  1 , Ming Li  1 , Shu-Bin Lin  1 , Zheng-Liang Yang  1 , Teng-Yu Xu  1 , Jin-Huan Yang  1 , Jun Yin  2   3
Affiliations

Comprehensive Analysis of Transcriptional Expression of hsa-mir-21 Predicted Target Genes and Immune Characteristics in Kidney Renal Clear Cell Carcinoma

Da-Ming Xu et al. Int J Med Sci. .

Abstract

Background: To uncover advanced prognosis biomarkers in patient with kidney renal clear cell carcinoma (KIRC), our study was the first to make a comprehensive analysis of hsa-mir-21 predicted target genes and explore the immune characteristics in KIRC. Methods: In this study, the comprehensive analysis of hsa-mir-21 predicted target genes and immune characteristics in KIRC were analyzed via TIMER2.0, UALCAN, Metascape, Kaplan-Meier plotter, Human Protein Atlas, CancerSEA, JASPAR, GEPIA, R package: GSVA package (version 1.34.0) & immune infiltration algorithm (ssGSEA) and R package: RMS package (version 6.2-0) & SURVIVAL package (version 3.2-10). Results: Up-transcriptional expressions of RP2, NFIA, SPRY1 were significantly associated with favorable prognosis in KIRC, whereas that of TGFBI was markedly significantly to unfavorable prognosis. Additionally, RP2, NFIA, SPRY1 and TGFBI were significantly relevant to the immune infiltration in KIRC. Finally, ZNF263 was a common predicted transcription factor of RP2, NFIA, SPRY1 and TGFBI, which can as an independent indicator for prognosis in KIRC patients. Conclusions: Hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) and the common transcription factor ZNF263 could be the advanced prognosis biomarkers in KIRC patients.

Keywords: Hsa-mir-21; Immune Characteristics; Kidney Renal Clear Cell Carcinoma; Predicted Target Gene; Prediction Model; Prognosis; Transcription Factor.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Top 20 over-expressed miRNAs in KIRC.
Figure 2
Figure 2
Hsa-mir-21 expression profile in KIRC based on patient's race, gender and age. Up-expression of hsa-mir-21 predicted target genes in KIRC patients.
Figure 3
Figure 3
Transcriptional expression of hsa-mir-21 predicted target genes in KIRC (***: P < 0.001, **: P < 0.01, *: P < 0.05).
Figure 4
Figure 4
Predicted functional pathways of hsa-mir-21 up-expressed predicted target genes.
Figure 5
Figure 5
Expression of 14 up-expressed hsa-mir-21 predicted target genes in different cancer.
Figure 6
Figure 6
Overall survival of transcriptional expression of up-expressed hsa-mir-21 predicted target genes in KIRC patient.
Figure 7
Figure 7
Subcellular location and protein expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) in KIRC.
Figure 8
Figure 8
The association of transcriptional expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) and KIRC stages (***: P < 0.001, **: P < 0.01, *: P < 0.05).
Figure 9
Figure 9
The association of transcriptional expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) and KIRC grades (***: P < 0.001, **: P < 0.01, *: P < 0.05).
Figure 10
Figure 10
The association of transcriptional expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) and KIRC nodal metastasis status (***: P < 0.001, **: P < 0.01, *: P < 0.05).
Figure 11
Figure 11
Single cell analysis of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) in normal kidney tissue.
Figure 12
Figure 12
The association of expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) and immune infiltration level in KIRC.
Figure 13
Figure 13
The correlation of expression of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI) with immune infiltration in KIRC.
Figure 14
Figure 14
The associated cancer functional states of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI).
Figure 15
Figure 15
The Nomogram of KIRC,
Figure 16
Figure 16
Transcription factor prediction and DNA base change of hsa-mir-21 predicted target genes (RP2, NFIA, SPRY1 and TGFBI).
Figure 17
Figure 17
Transcription factor ZNF263 as an independent indicator for prognosis in KIRC patients.
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