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. 2022 Aug 8;19(9):1399-1407.
doi: 10.7150/ijms.72565. eCollection 2022.

Effects of general anaesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats

Liyun Piao  1 , Og-Heui Na  2 , Eun-Hye Seo  3 , Seung-Wan Hong  4 , Kyo-Min Sohn  4 , Yubi Kwon  4 , Seung-Hyun Lee  5   6 , Seong-Hyop Kim  1   4   6
Affiliations

Effects of general anaesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats

Liyun Piao et al. Int J Med Sci. .

Abstract

Background: We hypothesized that the expression of exosomes under general anaesthesia with an inhalational anaesthetic agent would be changed. The study was designed to confirm the effect of general anesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats. Methods: After intraperitoneal administration for the mixture of ketamine and xylazine, tracheal intubation was performed. Just before the connection to ventilator, Control group and Anaesthesia group, according to anaesthesia with isoflurane, were allocated. The expressions of exosomes were checked in bronchoalveolar lavage (BAL), the blood and the tissues from the lung and the brain. Cytokines in the blood were also assessed. Results: The expressions of cluster of differentiation (CD)63 and CD81 as markers for the exosomes in the blood was increased after anaesthesia with isoflurane (CD63, 0.078 ± 0.057 % in Control group vs. 0.180 ± 0.036 % in Anaesthesia group, p = 0.02; CD81, 0.028 ± 0.034 % in Control group vs. 0.245 ± 0.054 % in Anaesthesia group, p < 0.01). However, the increased expression of them were not checked in BAL, and the tissues from the lung and the brain. The cytokines in the blood did not show any significant difference before and after anaesthesia with isoflurane. Conclusion: General anaesthesia with an inhalational anaesthetic agent increased the expression of exosomes in the blood. However, the change was limited in the blood, not the alveoli and the brain.

Keywords: CD63; CD81; Exosome; General anaesthesia; Isoflurane.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
The expression of exosomes in bronchoalveolar lavage. (A) Cluster of differentiation (CD) 63 (p = 0.38), (B) CD81 (p = 0.81).
Figure 2
Figure 2
The expression of exosomes in blood. (A) Cluster of differentiation (CD) 63, (B) CD81. *p < 0.05 compared with Control group.
Figure 3
Figure 3
The expression of exosomes in lung tissue. (A) Cluster of differentiation (CD) 63 (p = 0.12), (B) CD81 (p = 0.24). The arrows indicate CD63 or CD81.
Figure 4
Figure 4
The expression of exosomes in brain tissue. (A) Cluster of differentiation (CD) 63 (p = 0.32), (B) CD81 (p = 0.74). The arrows indicate CD63 or CD81.
Figure 5
Figure 5
Exosomes in electron microscopy. (A) Scale bar, 500 nm, (B) Scale bar, 200 nm. The arrows indicate exosomes.
See this image and copyright information in PMC

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