Neuroprotective Effects of Probiotic Lactobacillus reuteri GMNL-263 in the Hippocampus of Streptozotocin-Induced Diabetic Rats

Jing-Ying Lin¹, Bruce Chi-Kang Tsai², Hui-Chuan Kao³, Chien-Yi Chiang², Yun-An Chen⁴, William Shao-Tsu Chen^{5

6}, Tsung-Jung Ho^{7

8

9}, Chun-Hsu Yao^{10

11

12

13}, Wei-Wen Kuo^#^{4

14}, Chih-Yang Huang^#^{15

16

17

18

19}

Affiliations

¹ Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
² Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
³ Department of Public Health, Tzu Chi University, Hualien, Taiwan.
⁴ Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.
⁵ Department of Psychiatry, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁶ School of Medicine, Tzu Chi University, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁷ Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁸ Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁹ School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
¹⁰ Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
¹¹ School of Chinese Medicine, China Medical University, Taichung, Taiwan.
¹² Biomaterials Translational Research Center, China Medical University Hospital, Taichung, Taiwan.
¹³ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
¹⁴ Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.
¹⁵ Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁷ Center of General Education, Tzu Chi University of Science and Technology, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁸ Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁹ Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.

^# Contributed equally.

PMID: 36044175
DOI: 10.1007/s12602-022-09982-w

Neuroprotective Effects of Probiotic Lactobacillus reuteri GMNL-263 in the Hippocampus of Streptozotocin-Induced Diabetic Rats

Jing-Ying Lin et al. Probiotics Antimicrob Proteins. 2023 Oct.

. 2023 Oct;15(5):1287-1297.

doi: 10.1007/s12602-022-09982-w. Epub 2022 Aug 31.

Authors

Affiliations

¹ Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
² Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
³ Department of Public Health, Tzu Chi University, Hualien, Taiwan.
⁴ Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.
⁵ Department of Psychiatry, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁶ School of Medicine, Tzu Chi University, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁷ Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁸ Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁹ School of Post-Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
¹⁰ Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
¹¹ School of Chinese Medicine, China Medical University, Taichung, Taiwan.
¹² Biomaterials Translational Research Center, China Medical University Hospital, Taichung, Taiwan.
¹³ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
¹⁴ Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.
¹⁵ Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁷ Center of General Education, Tzu Chi University of Science and Technology, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁸ Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁹ Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.

^# Contributed equally.

PMID: 36044175
DOI: 10.1007/s12602-022-09982-w

Abstract

Diabetes-related brain complications have been reported in clinical patients and experimental models. The objective of the present study was to investigate the neuroprotective mechanisms of Lactobacillus reuteri GMNL-263 in streptozotocin (STZ)-induced diabetic rats. In this study, three different groups, namely control group, STZ-induced (55 mg/kg streptozotocin intraperitoneally) diabetic rats (DM), and DM rats treated with Lactobacillus reuteri GMNL-263 (1 × 10⁹ CFU/rat/day), were utilized to study the protective effect of GMNL-263 in the hippocampus of STZ-induced diabetic rats. The results demonstrated that GMNL-263 attenuated diabetes-induced hippocampal damage by enhancing the cell survival pathways and repressing both inflammatory and apoptotic pathways. Histopathological analysis revealed that GMNL-263 prevented structural changes in the hippocampus in the DM group and decreased the level of inflammation and apoptosis in the hippocampus of DM rats. The IGF1R cell survival signaling pathway also improved after GMNL-263 treatment. These results indicate that probiotic GMNL-263 exerts beneficial effects in the brain of diabetic rats and has potential ability for clinical application.

Keywords: Diabetic mellitus; Hyperglycemia; Lactic acid bacteria; Lactobacillus reuteri; Streptozotocin.

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References

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Neuroprotective Effects of Probiotic Lactobacillus reuteri GMNL-263 in the Hippocampus of Streptozotocin-Induced Diabetic Rats

Affiliations

Neuroprotective Effects of Probiotic Lactobacillus reuteri GMNL-263 in the Hippocampus of Streptozotocin-Induced Diabetic Rats

Authors

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Abstract

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