Preliminary investigation of the use of the enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of mycetoma
- PMID: 360443
- DOI: 10.1080/00362177885380301
Preliminary investigation of the use of the enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of mycetoma
Abstract
Antibody levels in a small group of Sudanese patients with clinically diagnosed mycetoma, and control groups were measured by countercurrent immunoelectrophoresis (CIE) and enzyme linked immunosorbent assay (ELISA). Antigens were prepared from the following organisms: Streptomyces somaliensis, Actinomadura madurae, A. pelletieri, Nocardia brasiliensis and Madurella mycetomi. Positive reactions were obtained in clinical cases against the homologous antigens in both CIE and ELISA; all heterologous and control sera negative in CIE but produced some reaction particularly against N. brasiliensis and M. mycetomi antigens in ELISA.
Similar articles
-
Comparison of antigens from agents of actinomycetoma by immunodiffusion and electrophoresis procedures.J Med Vet Mycol. 1990;28(5):363-71. doi: 10.1080/02681219080000471. J Med Vet Mycol. 1990. PMID: 2126557
-
A serological survey of antibodies to Streptomyces somaliensis and Actinomadura madurae in the Sudan enzyme linked immunosorbent assay (ELISA).Trans R Soc Trop Med Hyg. 1983;77(1):49-50. doi: 10.1016/0035-9203(83)90011-1. Trans R Soc Trop Med Hyg. 1983. PMID: 6679364
-
Enzyme-linked immunosorbent assay for serological diagnosis of Nocardia brasiliensis and clinical correlation with mycetoma infections.J Clin Microbiol. 1993 Nov;31(11):2901-6. doi: 10.1128/jcm.31.11.2901-2906.1993. J Clin Microbiol. 1993. PMID: 8263174 Free PMC article.
-
Mycetoma: reviewing a neglected disease.Clin Exp Dermatol. 2019 Mar;44(2):123-129. doi: 10.1111/ced.13642. Epub 2018 May 28. Clin Exp Dermatol. 2019. PMID: 29808607 Review.
-
Nocardia, nocardiosis and mycetoma.Med Mycol. 1998;36 Suppl 1:26-37. Med Mycol. 1998. PMID: 9988489 Review.
Cited by
-
Rapid bead counting and dispensing device for radioimmunoassays and enzyme-linked immunosorbent assays.J Clin Microbiol. 1981 Apr;13(4):801-4. doi: 10.1128/jcm.13.4.801-804.1981. J Clin Microbiol. 1981. PMID: 7014629 Free PMC article.
-
Mycetoma: Development of Diagnosis and Treatment.J Fungi (Basel). 2022 Jul 19;8(7):743. doi: 10.3390/jof8070743. J Fungi (Basel). 2022. PMID: 35887499 Free PMC article. Review.
-
Merits and pitfalls of currently used diagnostic tools in mycetoma.PLoS Negl Trop Dis. 2014 Jul 3;8(7):e2918. doi: 10.1371/journal.pntd.0002918. eCollection 2014 Jul. PLoS Negl Trop Dis. 2014. PMID: 24992636 Free PMC article. Review.
-
Mycetoma laboratory diagnosis: Review article.PLoS Negl Trop Dis. 2017 Aug 24;11(8):e0005638. doi: 10.1371/journal.pntd.0005638. eCollection 2017 Aug. PLoS Negl Trop Dis. 2017. PMID: 28837657 Free PMC article. Review.
-
Host genetic susceptibility to mycetoma.PLoS Negl Trop Dis. 2020 Apr 30;14(4):e0008053. doi: 10.1371/journal.pntd.0008053. eCollection 2020 Apr. PLoS Negl Trop Dis. 2020. PMID: 32352976 Free PMC article.