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. 2022 Aug 31;19(4):400-407.
doi: 10.4274/tjps.galenos.2021.65087.

In Vitro Anti-Aging Potential Evaluation of Maclura pomifera (Rafin.) Schneider 80% Methanol Extract with Quantitative HPTLC Analysis

Timur Hakan Barak  1 , İnci Kurt Celep  2 , Tuğba Buse Şentürk  1 , Hilal Bardakcı  1 , Engin Celep  2
Affiliations

In Vitro Anti-Aging Potential Evaluation of Maclura pomifera (Rafin.) Schneider 80% Methanol Extract with Quantitative HPTLC Analysis

Timur Hakan Barak et al. Turk J Pharm Sci. .

Abstract

Objectives: Maclura pomifera (Rafin.) Schneider is a widespread species all around the world, which is also frequently cultured for ornamental purposes. Previous studies revealed that M. pomifera fruits are rich in prenylated isoflavonoids, exhibit noteworthy biological activities, and have probable benefits, particularly, when applied topically. Considering that phenolic compounds are important sources in the development of anti-aging cosmetic products, this study investigated the anti-aging potential of M. pomifera 80% methanolic extract (MPM) by evaluating antioxidant and extracellular matrix degrading enzymes inhibiting activity.

Materials and methods: For this study, the inhibitory potential of 80% MPM against different enzymes associated with the aging process was evaluated. Given the unequivocal role of oxidative stress in aging, in vitro antioxidant tests were employed as well. Moreover, osajin was determined as the major bioactive isoflavonoid of the sample by high performance thin layer chromatography analysis.

Results: Results of the mechanistically different antioxidant assays exhibited notable antioxidant potential of the extract. Inhibition potential of MPM against hyaluronidase, collagenase, and elastase enzymes, which are directly linked to acceleration of the aging process, was investigated and results revealed that MPM inhibited the aforementioned enzymes explicitly. MPM had notable phenolic and flavonoid content; 113.92 ± 2.26 mg gallic acid equivalent/g and 66.41 ± 0.74 mg QE/g, respectively. When total antioxidant capacity assays were considered, it is possible to suggest that MPM is a promising anti-aging agent.

Conclusion: As a result, this study discloses that extracts of fruits of M. pomifera have significant anti-aging potential and may be used for this purpose.

Keywords: HPTLC; Maclura pomifera; anti-aging; antioxidants; osajin.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
(A) HPTLC chromatogram of standard osajin and test samples at 280 nm. (B) Overlapped UV spectra osajin and Maclura pomifera extract. (C) HPTLC chromatogram of standard osajin and test samples at 280 nm HPTLC: High performance thin layer chromatography, UV: Ultraviolet
Figure 2
Figure 2
(A) The percent anti-collagenase activity of MPM at a 1 mg/mL concentration at the end of 20 and 40 minutes is presented. In the statistical analysis, the p≤0.01 value was symbolized with **. % of Anti-elastase activity of 1 mg/mL MPM was shown in (B), and **** was meant p≤0.0001. EGCG was used as positive control in anti-collagenase and anti-elastase assays (A and B). (C) was shown that % of the anti-hyaluronidase activity of 1 mg/mL MPM at two different time points (at the end of the 40 and 80 minutes). p≤0.001 value was symbolized with *** MPM: Maclura pomifera 80% methanolic extract, EGCG: Epigallocatechin gallate
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