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. 2022:29:40.
doi: 10.1051/parasite/2022041. Epub 2022 Sep 1.

Enterocytozoon bieneusi in patients with diarrhea and in animals in the northeastern Chinese city of Yichun: genotyping and assessment of potential zoonotic transmission

Kexin Zhou  1 , Mingchao Liu  2 , Yanchen Wu  1 , Ran Zhang  2 , Ru Wang  2 , Hui Xu  2 , Yujia Wang  2 , Lan Yao  1 , Hongmei Yu  2 , Aiqin Liu  1
Affiliations

Enterocytozoon bieneusi in patients with diarrhea and in animals in the northeastern Chinese city of Yichun: genotyping and assessment of potential zoonotic transmission

Kexin Zhou et al. Parasite. 2022.

Abstract
in English, French

Enterocytozoon bieneusi is a common microsporidia species in humans and animals. Due to lack of effective vaccines and drugs, understanding of its epidemiological status and characteristics in different hosts is an important step in controlling the infection. The present study aimed at determining the prevalence of E. bieneusi in humans with diarrhea and animals in Yichun, in northeastern China, and assessing the epidemiological role of animals in the transmission of microsporidiosis. A total of 540 fecal samples were collected from diarrheal patients (n = 222) and 11 animal species (n = 318). Enterocytozoon bieneusi was identified and genotyped by polymerase chain reaction (PCR) and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. Enterocytozoon bieneusi was detected in 1.4% (3/222) of diarrheal patients, and genotype D and novel genotypes YCHH1 and YCHH2 were identified. Enterocytozoon bieneusi was detected in wild boars (7.7%), sika deer (8.2%), dogs (3.2%), and ostriches (10.7%), and genotypes D, Type IV, Peru6, BEB6 and novel genotypes YCHA1, YCHA2 and YCHA3 were identified. Genotypes YCHH1, YCHH2 and YCHA1 were phylogenetically assigned to group 1, while YCHA2 and YCHA3 to groups 2 and 11, respectively. The finding of genotype D in humans and animals, and the identification of zoonotic genotypes Peru6, Type IV, BEB6 in animal-derived E. bieneusi isolates indicate the potential of zoonotic transmission of microsporidiosis in the investigated area. The observation of the three novel genotypes in group 1 indicates their zoonotic potential.

Title: Enterocytozoon bieneusi chez des patients souffrant de diarrhée et des animaux dans la ville de Yichun, au nord-est de la Chine: génotypage et évaluation de la transmission zoonotique potentielle.

Abstract: Enterocytozoon bieneusi est une espèce de microsporidie commune chez les humains et les animaux. En raison du manque de vaccins et de médicaments efficaces, la compréhension de son statut épidémiologique et de ses caractéristiques chez différents hôtes est une étape importante dans le contrôle de l’infection. La présente étude visait à déterminer la prévalence d’E. bieneusi chez les humains souffrant de diarrhée et les animaux à Yichun, dans le nord-est de la Chine, et à évaluer le rôle épidémiologique des animaux dans la transmission de la microsporidiose. Cinq cent quarante échantillons fécaux ont été prélevés chez des patients diarrhéiques (n = 222) et 11 espèces animales (n = 318). Enterocytozoon bieneusi a été identifié et génotypé par PCR et séquençage de la région de l’espaceur interne transcrit (ITS) du gène de l’ARNr. Enterocytozoon bieneusi a été détecté chez 1,4 % (3/222) des patients souffrant de diarrhée, et le génotype D et les nouveaux génotypes YCHH1 et YCHH2 ont été identifiés. Enterocytozoon bieneusi a été détecté chez des sangliers (7,7 %), des cerfs sika (8,2 %), des chiens (3,2 %) et des autruches (10,7 %), et les génotypes D, Type IV, Peru6, BEB6 et les nouveaux génotypes YCHA1, YCHA2 et YCHA3 ont été identifiés. Les génotypes YCHH1, YCHH2 et YCHA1 ont été phylogénétiquement assignés au groupe 1, et YCHA2 et YCHA3 respectivement aux groupes 2 et 11. La découverte du génotype D chez les humains et les animaux et l’identification des génotypes zoonotiques Peru6, Type IV, BEB6 dans les isolats d’E. bieneusi d’origine animale indiquent le potentiel de transmission zoonotique de la microsporidiose dans la zone étudiée. L’observation des trois nouveaux génotypes dans le groupe 1 indique leur potentiel zoonotique.

Keywords: Animals; Diarrheal patients; Enterocytozoon bieneusi; Genotyping; ITS region.

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Figures

Figure 1
Figure 1
Phylogenetic relationships of the E. bieneusi genotypes of E. bieneusi identified in this study and other known genotypes as inferred by a neighbor-joining analysis of ITS sequences based on genetic distances calculated by the Kimura 2-parameter model. The numbers on the branches are percent bootstrapping values from 1000 replicates. Each nucleotide sequence is identified by its accession number, host origin, and genotype designation. The black triangles and circles are known and novel genotypes identified in this study, respectively.
See this image and copyright information in PMC

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