Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan;25(1):11-16.
doi: 10.1111/1462-2920.16143. Epub 2022 Aug 30.

Is it possible to predict signal molecules that are recognized by bacterial receptors?

Miguel A Matilla  1 , Elizabet Monteagudo-Cascales  1 , Jean Paul Cerna-Vargas  1   2 , Vadim M Gumerov  3 , Igor B Zhulin  3 , Tino Krell  1
Affiliations

Is it possible to predict signal molecules that are recognized by bacterial receptors?

Miguel A Matilla et al. Environ Microbiol. 2023 Jan.
No abstract available

PubMed Disclaimer

Figures

Fig. 1)
Fig. 1). Sensor domains that preferentially recognize a single molecule or families of closely related molecules.
Reproduced with permission from (Matilla et al., 2022a).
Fig. 2)
Fig. 2). Conserved sequence motif in the ligand binding pocket of amino acid-binding dCache domains.
A) The consensus motif. Numbers above the motif correspond to positions in the P. aeruginosa PctA chemoreceptor. B) Zoom at the binding pocket of the sensor domain of the PctA chemoreceptor in complex with bound L-Trp. The amino acids that interact with L-Trp are shown in the same colour mode as in panel A. C) Isothermal titration calorimetry study of L-Ala binding to the PctA sensor domain and mutants in individual amino acids of the motif. Upper panel: raw titration data. Lower panel: Best fit of binding data for the wild type protein. Modified figure reproduced with permission from (Gumerov et al., 2022).
Fig. 3)
Fig. 3). Experimental verification of ligand binding to sensor domains predicted to recognize amino acids.
Isothermal titration calorimetry studies of individual sensor domains with different amino acids. The receptor family and corresponding bacterial species are indicated. Modified figure reproduced with permission from (Gumerov et al., 2022).
See this image and copyright information in PMC

References

    1. Cheung J, and Hendrickson WA (2008) Crystal structures of C4-dicarboxylate ligand complexes with sensor domains of histidine kinases DcuS and DctB. J Biol Chem 283: 30256–30265. - PMC - PubMed
    1. Colin R, Ni B, Laganenka L, and Sourjik V (2021) Multiple functions of flagellar motility and chemotaxis in bacterial physiology. FEMS Microbiol Rev 45: fuab038. - PMC - PubMed
    1. Corral-Lugo A, Matilla MA, Martin-Mora D, Silva Jimenez H, Mesa Torres N, Kato J et al. (2018) High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa. MBio 9: e01894–01818. - PMC - PubMed
    1. Elgamoudi BA, Andrianova EP, Shewell LK, Day CJ, King RM, Taha et al. (2021) The Campylobacter jejuni chemoreceptor Tlp10 has a bimodal ligand-binding domain and specificity for multiple classes of chemoeffectors. Sci Signal 14: eabc8521. - PMC - PubMed
    1. Fernandez M, Morel B, Corral-Lugo A, and Krell T (2016) Identification of a chemoreceptor that specifically mediates chemotaxis toward metabolizable purine derivatives. Mol Microbiol 99: 34–42. - PubMed

Publication types