Detection and localization of early- and late-stage cancers using platelet RNA

doi:10.1016/j.ccell.2022.08.006

. 2022 Sep 12;40(9):999-1009.e6.

doi: 10.1016/j.ccell.2022.08.006. Epub 2022 Sep 1.

Detection and localization of early- and late-stage cancers using platelet RNA

Sjors G J G In 't Veld¹, Mohammad Arkani², Edward Post³, Mafalda Antunes-Ferreira³, Silvia D'Ambrosi³, Daan C L Vessies⁴, Lisa Vermunt⁵, Adrienne Vancura³, Mirte Muller⁶, Anna-Larissa N Niemeijer⁷, Jihane Tannous⁸, Laura L Meijer⁹, Tessa Y S Le Large⁹, Giulia Mantini¹⁰, Niels E Wondergem¹¹, Kimberley M Heinhuis¹², Sandra van Wilpe¹³, A Josien Smits⁷, Esther E E Drees¹⁴, Eva Roos¹⁵, Cyra E Leurs¹⁶, Lee-Ann Tjon Kon Fat¹⁷, Ewoud J van der Lelij³, Govert Dwarshuis³, Maarten J Kamphuis³, Lisanne E Visser³, Romee Harting³, Annemijn Gregory³, Markus W Schweiger¹⁸, Laurine E Wedekind³, Jip Ramaker³, Kenn Zwaan³, Heleen Verschueren³, Idris Bahce⁷, Adrianus J de Langen⁶, Egbert F Smit⁶, Michel M van den Heuvel¹⁹, Koen J Hartemink²⁰, Marijke J E Kuijpers²¹, Mirjam G A Oude Egbrink²², Arjan W Griffioen¹⁰, Rafael Rossel²³, T Jeroen N Hiltermann²⁴, Elizabeth Lee-Lewandrowski²⁵, Kent B Lewandrowski²⁵, Philip C De Witt Hamer³, Mathilde Kouwenhoven²⁶, Jaap C Reijneveld²⁷, William P J Leenders²⁸, Ann Hoeben²⁹, Irma M Verdonck-de Leeuw³⁰, C René Leemans³¹, Robert J Baatenburg de Jong³², Chris H J Terhaard³³, Robert P Takes³⁴, Johannes A Langendijk³⁵, Saskia C de Jager³⁶, Adriaan O Kraaijeveld³⁷, Gerard Pasterkamp³⁶, Minke Smits¹³, Jack A Schalken³⁸, Sylwia Łapińska-Szumczyk³⁹, Anna Łojkowska³⁹, Anna J Żaczek⁴⁰, Henk Lokhorst⁴¹, Niels W C J van de Donk⁴¹, Inger Nijhof⁴¹, Henk-Jan Prins⁴¹, Josée M Zijlstra⁴¹, Sander Idema³, Johannes C Baayen³, Charlotte E Teunissen⁵, Joep Killestein¹⁶, Marc G Besselink¹⁵, Lindsay Brammen⁴², Thomas Bachleitner-Hofmann⁴³, Farrah Mateen⁴⁴, John T M Plukker⁴⁵, Michal Heger⁴⁶, Quirijn de Mast⁴⁷, Ton Lisman⁴⁸, D Michiel Pegtel¹⁴, Harm-Jan Bogaard⁷, Jacek Jassem⁴⁹, Anna Supernat⁴⁰, Niven Mehra¹³, Winald Gerritsen¹³, Cornelis D de Kroon⁵⁰, Christianne A R Lok⁵¹, Jurgen M J Piek⁵², Neeltje Steeghs¹², Winan J van Houdt⁵³, Ruud H Brakenhoff¹¹, Gabe S Sonke⁵⁴, Henk M Verheul¹³, Elisa Giovannetti⁵⁵, Geert Kazemier⁹, Siamack Sabrkhany⁵⁶, Ed Schuuring⁵⁷, Erik A Sistermans⁵⁸, Rob Wolthuis⁵⁹, Hanne Meijers-Heijboer⁵⁹, Josephine Dorsman⁵⁹, Cees Oudejans⁶⁰, Bauke Ylstra¹⁴, Bart A Westerman³, Daan van den Broek⁴, Danijela Koppers-Lalic³, Pieter Wesseling⁶¹, R Jonas A Nilsson¹⁷, W Peter Vandertop³, David P Noske³, Bakhos A Tannous⁸, Nik Sol²⁶, Myron G Best⁶², Thomas Wurdinger⁶³

Affiliations

¹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Neurochemistry Lab, Boelelaan 1117, Amsterdam, the Netherlands; Neuroscience Campus Amsterdam, Amsterdam, the Netherlands.
² Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pulmonary Medicine, Boelelaan 1117, Amsterdam, the Netherlands.
³ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands.
⁴ Department of Laboratory Medicine, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Neurochemistry Lab, Boelelaan 1117, Amsterdam, the Netherlands; Neuroscience Campus Amsterdam, Amsterdam, the Netherlands.
⁶ Department of Thoracic Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁷ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pulmonary Medicine, Boelelaan 1117, Amsterdam, the Netherlands.
⁸ Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Neuroscience Program, Harvard Medical School, Boston, MA, USA.
⁹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁰ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Medical Oncology, Boelelaan 1117, Amsterdam, the Netherlands.
¹¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹² Department of Medical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands; Department of Clinical Pharmacology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
¹³ Department of Medical Oncology, Radboud University Medical Center, Nijmegen, the Netherlands.
¹⁴ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pathology, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁵ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁶ Neuroscience Campus Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands; MS Center Amsterdam, Amsterdam, the Netherlands.
¹⁷ Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.
¹⁸ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Neuroscience Program, Harvard Medical School, Boston, MA, USA.
¹⁹ Department of Thoracic Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands; Department of Respiratory Diseases, Radboud University Medical Center, Nijmegen, the Netherlands.
²⁰ Department of Thoracic Surgery, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
²¹ Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands; Thrombosis Expertise Centre, Heart and Vascular Centre, Maastricht University Medical Centre, Maastricht, the Netherlands.
²² Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
²³ Translational Research Unit, Dr. Rosell Oncology Institute, Quirón Dexeus University Hospital, Barcelona, Spain; Pangaea Biotech SL, Barcelona, Spain; Catalan Institute of Oncology, Hospital Germans Trias i Pujol, Barcelona, Spain; Molecular Oncology Research (MORe) Foundation, Barcelona, Spain.
²⁴ University of Groningen, Department of Pulmonary Diseases, University Medical Center Groningen, Groningen, the Netherlands.
²⁵ Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
²⁶ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands.
²⁷ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands; Department of Neurology, Stichting Epilepsie Instellingen Nederland (SEIN), Heemstede, the Netherlands.
²⁸ Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands.
²⁹ Department of Medical Oncology, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Center, Maastricht, the Netherlands.
³⁰ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands; Department of Clinical, Neuro- and Developmental Psychology, Faculty of Behavioral and Movement Sciences & Amsterdam Public Health Research Institute, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
³¹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
³² Department of Otolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, the Netherlands.
³³ Department of Radiotherapy, University Medical Center Utrecht, Utrecht, the Netherlands.
³⁴ Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center, Nijmegen, the Netherlands.
³⁵ Department of Radiation Oncology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
³⁶ Department of Experimental Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands.
³⁷ Department of Cardiology, Division of Heart and Lungs, Utrecht University Medical Center, Utrecht, the Netherlands.
³⁸ Urological Research Laboratory, Radboud University Medical Center, Nijmegen, the Netherlands; Department of Urology, Radboud University Medical Center, Nijmegen, the Netherlands.
³⁹ Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk, Gdańsk, Poland.
⁴⁰ Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
⁴¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Hematology, Boelelaan 1117, Amsterdam, the Netherlands.
⁴² Department of Surgery, Division of General Surgery, Medical University of Vienna, Vienna, Austria.
⁴³ Clinical Institute of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
⁴⁴ Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
⁴⁵ Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁴⁶ Department of Pharmaceutics, Jiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, College of Medicine, Jiaxing University, Jiaxing, Zhejiang, PR China; Department of Pathology, Laboratory Experimental Oncology, Erasmus MC, Rotterdam, the Netherlands.
⁴⁷ Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the Netherlands.
⁴⁸ Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands; Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁴⁹ Department of Oncology and Radiotherapy, Medical University of Gdańsk, Gdańsk, Poland.
⁵⁰ Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, the Netherlands.
⁵¹ Department of Gynaecological Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek, Amsterdam, the Netherlands; Center of Gynaecologic Oncology Amsterdam, the Netherlands Cancer Institute - Antoni van Leeuwenhoek, Amsterdam, the Netherlands.
⁵² Department of Obstetrics and Gynaecology and Catharina Cancer Institute, Catharina Hospital, Eindhoven, the Netherlands.
⁵³ Department of Surgical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵⁴ Department of Medical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵⁵ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Medical Oncology, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Pharmacology Lab, AIRC Start-Up Unit, Fondazione Pisana per La Scienza, Pisa, Italy.
⁵⁶ Department of Physiology, Maastricht University, Maastricht, the Netherlands.
⁵⁷ Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁵⁸ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Genetics, Boelelaan 1117, Amsterdam, the Netherlands; Amsterdam Reproduction & Development Research Institute, Amsterdam, the Netherlands.
⁵⁹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Genetics, Boelelaan 1117, Amsterdam, the Netherlands.
⁶⁰ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Boelelaan 1117, Amsterdam, the Netherlands.
⁶¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pathology, Boelelaan 1117, Amsterdam, the Netherlands; Department of Pathology, Princess Máxima Center for Pediatric Oncology and University Medical Center Utrecht, Utrecht, the Netherlands.
⁶² Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands. Electronic address: m.g.best@amsterdamumc.nl.
⁶³ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands. Electronic address: t.wurdinger@amsterdamumc.nl.

PMID: 36055228
DOI: 10.1016/j.ccell.2022.08.006

Free article

Detection and localization of early- and late-stage cancers using platelet RNA

Sjors G J G In 't Veld et al. Cancer Cell. 2022.

Free article

. 2022 Sep 12;40(9):999-1009.e6.

doi: 10.1016/j.ccell.2022.08.006. Epub 2022 Sep 1.

Authors

Affiliations

¹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Neurochemistry Lab, Boelelaan 1117, Amsterdam, the Netherlands; Neuroscience Campus Amsterdam, Amsterdam, the Netherlands.
² Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pulmonary Medicine, Boelelaan 1117, Amsterdam, the Netherlands.
³ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands.
⁴ Department of Laboratory Medicine, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Neurochemistry Lab, Boelelaan 1117, Amsterdam, the Netherlands; Neuroscience Campus Amsterdam, Amsterdam, the Netherlands.
⁶ Department of Thoracic Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁷ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pulmonary Medicine, Boelelaan 1117, Amsterdam, the Netherlands.
⁸ Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Neuroscience Program, Harvard Medical School, Boston, MA, USA.
⁹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁰ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Medical Oncology, Boelelaan 1117, Amsterdam, the Netherlands.
¹¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹² Department of Medical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands; Department of Clinical Pharmacology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
¹³ Department of Medical Oncology, Radboud University Medical Center, Nijmegen, the Netherlands.
¹⁴ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pathology, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁵ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
¹⁶ Neuroscience Campus Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands; MS Center Amsterdam, Amsterdam, the Netherlands.
¹⁷ Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.
¹⁸ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Neuroscience Program, Harvard Medical School, Boston, MA, USA.
¹⁹ Department of Thoracic Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands; Department of Respiratory Diseases, Radboud University Medical Center, Nijmegen, the Netherlands.
²⁰ Department of Thoracic Surgery, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
²¹ Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands; Thrombosis Expertise Centre, Heart and Vascular Centre, Maastricht University Medical Centre, Maastricht, the Netherlands.
²² Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
²³ Translational Research Unit, Dr. Rosell Oncology Institute, Quirón Dexeus University Hospital, Barcelona, Spain; Pangaea Biotech SL, Barcelona, Spain; Catalan Institute of Oncology, Hospital Germans Trias i Pujol, Barcelona, Spain; Molecular Oncology Research (MORe) Foundation, Barcelona, Spain.
²⁴ University of Groningen, Department of Pulmonary Diseases, University Medical Center Groningen, Groningen, the Netherlands.
²⁵ Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
²⁶ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands.
²⁷ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurology, Boelelaan 1117, Amsterdam, the Netherlands; Department of Neurology, Stichting Epilepsie Instellingen Nederland (SEIN), Heemstede, the Netherlands.
²⁸ Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands.
²⁹ Department of Medical Oncology, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Center, Maastricht, the Netherlands.
³⁰ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands; Department of Clinical, Neuro- and Developmental Psychology, Faculty of Behavioral and Movement Sciences & Amsterdam Public Health Research Institute, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
³¹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Otolaryngology and Head and Neck Surgery, Boelelaan 1117, Amsterdam, the Netherlands.
³² Department of Otolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, the Netherlands.
³³ Department of Radiotherapy, University Medical Center Utrecht, Utrecht, the Netherlands.
³⁴ Department of Otorhinolaryngology and Head and Neck Surgery, Radboud University Medical Center, Nijmegen, the Netherlands.
³⁵ Department of Radiation Oncology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
³⁶ Department of Experimental Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands.
³⁷ Department of Cardiology, Division of Heart and Lungs, Utrecht University Medical Center, Utrecht, the Netherlands.
³⁸ Urological Research Laboratory, Radboud University Medical Center, Nijmegen, the Netherlands; Department of Urology, Radboud University Medical Center, Nijmegen, the Netherlands.
³⁹ Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk, Gdańsk, Poland.
⁴⁰ Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
⁴¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Hematology, Boelelaan 1117, Amsterdam, the Netherlands.
⁴² Department of Surgery, Division of General Surgery, Medical University of Vienna, Vienna, Austria.
⁴³ Clinical Institute of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
⁴⁴ Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
⁴⁵ Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁴⁶ Department of Pharmaceutics, Jiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, College of Medicine, Jiaxing University, Jiaxing, Zhejiang, PR China; Department of Pathology, Laboratory Experimental Oncology, Erasmus MC, Rotterdam, the Netherlands.
⁴⁷ Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the Netherlands.
⁴⁸ Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands; Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁴⁹ Department of Oncology and Radiotherapy, Medical University of Gdańsk, Gdańsk, Poland.
⁵⁰ Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, the Netherlands.
⁵¹ Department of Gynaecological Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek, Amsterdam, the Netherlands; Center of Gynaecologic Oncology Amsterdam, the Netherlands Cancer Institute - Antoni van Leeuwenhoek, Amsterdam, the Netherlands.
⁵² Department of Obstetrics and Gynaecology and Catharina Cancer Institute, Catharina Hospital, Eindhoven, the Netherlands.
⁵³ Department of Surgical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵⁴ Department of Medical Oncology, the Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands.
⁵⁵ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Medical Oncology, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Pharmacology Lab, AIRC Start-Up Unit, Fondazione Pisana per La Scienza, Pisa, Italy.
⁵⁶ Department of Physiology, Maastricht University, Maastricht, the Netherlands.
⁵⁷ Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
⁵⁸ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Genetics, Boelelaan 1117, Amsterdam, the Netherlands; Amsterdam Reproduction & Development Research Institute, Amsterdam, the Netherlands.
⁵⁹ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Genetics, Boelelaan 1117, Amsterdam, the Netherlands.
⁶⁰ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Clinical Chemistry, Boelelaan 1117, Amsterdam, the Netherlands.
⁶¹ Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands; Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Pathology, Boelelaan 1117, Amsterdam, the Netherlands; Department of Pathology, Princess Máxima Center for Pediatric Oncology and University Medical Center Utrecht, Utrecht, the Netherlands.
⁶² Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands. Electronic address: m.g.best@amsterdamumc.nl.
⁶³ Amsterdam UMC Location Vrije Universiteit Amsterdam, Department of Neurosurgery, Boelelaan 1117, Amsterdam, the Netherlands; Cancer Center Amsterdam and Liquid Biopsy Center, Amsterdam, the Netherlands; Brain Tumor Center Amsterdam, Amsterdam, the Netherlands. Electronic address: t.wurdinger@amsterdamumc.nl.

PMID: 36055228
DOI: 10.1016/j.ccell.2022.08.006

Abstract

Cancer patients benefit from early tumor detection since treatment outcomes are more favorable for less advanced cancers. Platelets are involved in cancer progression and are considered a promising biosource for cancer detection, as they alter their RNA content upon local and systemic cues. We show that tumor-educated platelet (TEP) RNA-based blood tests enable the detection of 18 cancer types. With 99% specificity in asymptomatic controls, thromboSeq correctly detected the presence of cancer in two-thirds of 1,096 blood samples from stage I-IV cancer patients and in half of 352 stage I-III tumors. Symptomatic controls, including inflammatory and cardiovascular diseases, and benign tumors had increased false-positive test results with an average specificity of 78%. Moreover, thromboSeq determined the tumor site of origin in five different tumor types correctly in over 80% of the cancer patients. These results highlight the potential properties of TEP-derived RNA panels to supplement current approaches for blood-based cancer screening.

Keywords: RNA; TEP; blood; blood platelets; cancer; early detection; liquid biopsy.

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Conflict of interest statement

Declaration of interests M.G. Best, R.J.A.N., and T.W. are inventors on relevant patent applications (PCT/NL2011/050518 and PCT/NL2018/050110). R.J.A.N. and T.W. are shareholders of Illumina, Inc. M.H. is chief formulation officer at Nurish.Me, Inc., and Camelina Sun LLC and has equity in those companies (whose business activities are unrelated to the present work). D.M.P. and D.K.L. are shareholders of ExBiome BV.

Comment in

A new method for early cancer detection based on platelet transcriptomics will have low positive predictive value.
Diamandis EP. Diamandis EP. Clin Chem Lab Med. 2022 Dec 2;61(6):e85-e86. doi: 10.1515/cclm-2022-1025. Print 2023 May 25. Clin Chem Lab Med. 2022. PMID: 36472964 No abstract available.

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Detection and localization of early- and late-stage cancers using platelet RNA

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Detection and localization of early- and late-stage cancers using platelet RNA

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