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. 2022 Dec 15:852:158265.
doi: 10.1016/j.scitotenv.2022.158265. Epub 2022 Aug 31.

The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Eline F de Jonge  1 , Céline M Peterse  1 , Jaap M Koelewijn  1 , Anne-Merel R van der Drift  1 , Rudolf F H J van der Beek  1 , Erwin Nagelkerke  1 , Willemijn J Lodder  2
Affiliations

The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Eline F de Jonge et al. Sci Total Environ. .

Abstract
in English, Dutch

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As of 18 August 2022, 1087 confirmed cases of monkeypox are reported in the Netherlands. Monkeypox virus likely ends up in wastewater because i) skin flakes from areas affected by the typical rash and scabbing may wash off, and ii) monkeypox virus has been detected in animal and human feces. Here we describe a method to qualitatively detect monkeypox virus DNA in wastewater, that may prove a valuable surveillance tool for outbreaks.

Keywords: Environmental surveillance; Monkeypox virus; Public health; WBE; Wastewater.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Graphical abstract
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